Anti-δ1-Catenin (central region) Antibody (M354)

Name: Anti-δ1-Catenin (central region) Antibody
Brand: PhosphoSolutions
SKU: CM3541
Price: 360.0 USD
Availability: InStock

2 Citations

Our δ1-Catenin (central region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat δ1-Catenin (central region) and is Protein A purified. It is great for use in WB, ICC, IP.

Immunocytochemical labeling of δ1-Catenin in untreated (Top) or pervanadate-treated (bottom) A431 cells. The cells were labeled with mouse monoclonal δ1-Catenin (a.a. 275-285), δ1-Catenin (Tyr-228), δ1-Catenin (Tyr-280), or δ1-Catenin (Tyr-302) antibodies. The antibodies were detected using donkey anti-mouse secondary antibodies conjugated to Cy3.

Western blot analysis of δ1-Catenin phosphorylation in A431 cells stimulated with calyculin A (100 nM) for 30 min. (lanes 1 & 3) then the blot was treated with lambda phosphatase (lanes 2 & 4). The blots were probed with either mouse monoclonal anti-δ1-Catenin (a.a. 275-285) (lanes 1 & 2) or rabbit polyclonal anti-δ1-Catenin (Thr-916) (lanes 3 & 4).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IP, WB

Host Species

Mouse

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SKU: CM3541

Volume: 100 µL

Variant

Price:

$360.00

Quantity:

Product Details

Target

δ1-Catenin (central region)

Target Description

Catenins have emerged as molecular sensors that integrate cell-cell junctions and cytoskeletal dynamics with signaling pathways that control morphogenesis and cell to cell communication. δ1-Catenin (p120 catenin) is a catenin family member which contains an N-terminal coiled-coil domain, a regulatory domain containing multiple phosphorylation sites, and a central Armadillo repeat domain. δ1-Catenin regulates E-cadherin turnover, and has both positive and negative effects on cadherin-mediated adhesion. Actin dynamics are also regulated by δ1-Catenin, which can modulate RhoA, Rac and cdc42 activity. δ1-Catenin is phosphorylated at multiple tyrosine, serine and threonine sites both in vitro and in vivo. High levels of δ1-Catenin phosphorylated at Tyr-228 are commonly seen in several carcinoma cell lines and after EGFR activation. Many other tyrosine sites are also phosphorylated in the N-terminal region including Tyr-96, Tyr-112, Tyr-280, and Tyr-302. In addition, Thr-310 and Thr-916 are constituitively phosphorylated in many cell types, however this phosphorylation may occur only in δ1-Catenin associated with the plasma membrane.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M354

Isotype

IgG1

Applications

ICC, IP, WB

Host Species

Mouse

Gene Name

CTNND1

Molecular Weight

110

Antigen

Clone (M354) was generated from a peptide that includes amino acids from the central region of human δ1-Catenin. This peptide sequence is highly conserved in rat and mouse δ1-Catenin.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100
IP: 1:50

Conjugate

Unconjugated

Specificity

The antibody detects a 110 kDa* protein corresponding to the molecular mass of δ1-Catenin on SDS-PAGE immunoblots of human A431 and HUVEC cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

pp120 Src substrate, p120

UniProt Details

UniProt (Human): O60716
UniProt (Immunogen Species): O60716

Product Specific References for Applications and Species

Western Blot: Human

Western Blot: Human
PMID	Dilution	Publication
35660718	not listed	Chow, SE, et al. 2022. Nuclear p120 catenin is a component of the perichromosomal layer and coordinates sister chromatid segregation during mitosis in lung cancer cells. Cell death & disease, 526.
29549325	not listed	Yang, CT, et al. 2018. Downregulation of lumican accelerates lung cancer cell invasion through p120 catenin. Cell death & disease, 414.

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Anti-δ1-Catenin (central region) Antibody (M354)

SKU: CM3541

Product Details

Product Specific References for Applications and Species

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