Anti-CXCR4 (Ser-324/Ser-325), Phosphospecific Antibody

Name: Anti-CXCR4 (Ser-324/Ser-325), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: CP4251
Price: 329.0 USD
Availability: InStock

4 Citations

Our CXCR4 (Ser-324/Ser-325) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat CXCR4 (Ser-324/Ser-325) and is antigen affinity purified. It is great for use in WB, ICC, IHC.

Immunocytochemical labeling of CXCR4 in chick pluripotent cells. The cells were labeled with rabbit polyclonal CXCR4 (S324/S325) antibody (CP4251), then detected using appropriate secondary antibody (Red). (Image provided by Dr. Yangqing Lu at the Regenerative Bioscience Center, University of Georgia).

Western blot analysis of human Jurkat cells treated with 100 nM calyculin A for 30 min. then the blots were untreated (-) or treated (+) with lambda phosphatase. The blots were probed with rabbit polyclonals anti-CXCR4 (Ser-324/Ser-325) (left panel) or anti-CXCR4 (a.a. 322-329) (right panel).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Rabbit

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SKU: CP4251

Volume: 100 µL

Variant

Price:

$329.00

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Product Details

Target

CXCR4 (Ser-324/Ser-325)

Target Description

The chemokine receptor CXCR4 is a widely expressed G protein-coupled receptor required for development, hematopoiesis, organogenesis, and vascularization. In disease, CXCR4 has been implicated in WHIM syndrome, HIV, and cancers. Regulation of CXCR4 function occurs through phosphorylation at multiple sites in the C-terminal region. These sites have been shown to be phosphorylated after CXCL12 stimulation, and involve several kinases, such as PKC and GRK kinases. After CXCL12 stimulation of HEK293 cells, Ser-324 and Ser-325 become phosphorylated by PKC and GRK6, while Ser-330 and Ser-339 are phosphorylated by only GRK6. In human astroglia cells, Ser-324 and Ser-325 are rapidly phosphorylated in endogenous CXCR4, while Ser-330 was phosphorylated with slower kinetics. In addition, arrestin binding to CXCR4 is driven by this phosphorylation of far C-terminal residues. Thus, site-specific phosphorylation of CXCR4 may be regulated by multiple kinases lead to complex regulation of CXCR4 signaling.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Rabbit

Gene Name

CXCR4

Molecular Weight

35,42,50,100

Antigen

Phospho-CXCR4 (Ser-324/Ser-325) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-324 and Ser-325 in the C-terminal region of human CXCR4. This sequence is conserved in mouse, rat, and chicken CXCR4, but has low homology to other CXCR family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
IHC: 1:100
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects bands at 35, 42, 50, and 100 kDa* corresponding to various post-translationally modified forms of CXCR4 on SDS-PAGE immunoblots of human Jurkat and PC3 cells stimulated with calyculin A. These bands co-migrate with similar bands detected with anti-CXCR4 (a.a. 322-329) and anti-CXCR4 (a.a. 328-338) antibodies.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

S324/S325

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E; CXCR4

UniProt Details

UniProt (Human): P61073
UniProt (Immunogen Species): P61073

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Western Blot: Human

Immunohistochemistry: Mouse
PMID	Dilution	Publication
24154522	not listed	Yang, S, et al. 2013. Cxcl12/Cxcr4 signaling controls the migration and process orientation of A9-A10 dopaminergic neurons. Development, 4554-4564.

Western Blot: Human
PMID	Dilution	Publication
30327409	not listed	Hitchinson, B, et al. 2018. Biased antagonism of CXCR4 avoids antagonist tolerance. Science signaling, eaat2214.
27127886	not listed	Saint-Georges, S, et al. 2016. Protein kinase D-dependent CXCR4 down-regulation upon BCR triggering is linked to lymphadenopathy in chronic lymphocytic leukaemia. Oncotarget, 41031-41046.
25775124	1:1000	Panneerselvam, J, et al. 2015. IL-24 inhibits lung cancer cell migration and invasion by disrupting the SDF-1/CXCR4 signaling axis. PLoS One, e0122439.

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Anti-CXCR4 (Ser-324/Ser-325), Phosphospecific Antibody

SKU: CP4251

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-CXCR4 (Ser-324/Ser-325), Phosphospecific Antibody

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