Our CRMP2 (Thr-555) mouse monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat CRMP2 (Thr-555) and is antigen affinity purified. It is great for use in WB.
Western blot of rat PC12 cells stimulated with calyculin A (100 nM) for 30 min (lanes 1-4). Then the blot was treated with lambda phosphatase to dephosphorylate CRMP2 (lanes 2 & 4). The blot was probed with rabbit polyclonal anti-CRMP2 (C-terminal Region) antibody (lanes 1 & 2) or mouse monoclonal anti-CRMP2 (Thr-555) antibody (lanes 3 & 4).
CRMP2 (CRMP-62, TOAD-64, DRP-2) is a microtubule associated protein involved in neuron development and axon pathfinding. CRMP2 binds to tubulin heterodimers and promotes microtubule assembly. The overexpression of CRMP2 facilitates the rate of axonal growth, whereas the mutated form that lacks activity toward the microtubule assembly inhibits axonal growth in a dominant negative manner. Phosphorylation of CRMP2 regulates its activity and this type of regulation has been implicated in axon growth cone collapse induced by several repulsive cues. Cdk5 and GSK3 phosphorylation occurs downstream of the repulsive cue, Sema-3A. Several residues in CRMP2 are phosphorylated by GSK3 (Ser-518,Thr-514, and Thr-509), and a priming site (Ser-522). These sites are conserved in human CRMP1 and CRMP4, but not in CRMP3 or CRMP5. The priming site is also phosphorylated by Cdk5. In contrast, ROCK phosphorylates Thr-555 leading to LPA, MAG, or Ephrin-A5 mediated growth cone collapse. Thus, CRMP2 phosphorylation status may be a critical element of pathways that control axon pathfinding.
Antigen Affinity Purified
Monoclonal
M539
IgG1
WB
Mouse
DPYSL2
70
Clone M539 was generated from a phospho-CRMP2 (Thr-555) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Thr-555 in human CRMP2. This sequence is conserved in rat and mouse CRMP2 and the phospho-site is not conserved in other CRMP family members.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:500
Unconjugated
The antibody detects a 70 kDa* protein corresponding to CRMP2 on immunoblots of rat PC12 or mouse C2C12 cells treated with calyculin A. This reactivity is not observed after lambda phosphatase dephosphorylation of Thr-555.
Phosphorylated
Thr-555
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Li, Y, et al. 2022. CRMP2 Modulates Mossy Fiber Sprouting in Dentate Gyrus of Pilocarpine Induced Rat Model of Epilepsy. Biochemical and Biophysical Research Communications, 141-147.
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