Anti-Connexin-45 (Cx45) Antibody

Name: Anti-Connexin-45 (Cx45) Antibody
Brand: Biosensis
SKU: S-061-100
Price: 337.0 USD
Availability: InStock

Our Anti-Connexin-45 (Cx45) sheep polyclonal primary antibody detects human Connexin-45 (Cx45), and is whole serum. It is validated for use in WB.

B: Western Blotting of tissue extracts from rat brain, heart, lung, liver, caudal artery (CA) and thoracic aorta (ThA) using S-061-100. Arrows show the position of the expected Cx band. The left panels represent incubation with Cx antibody whereas right panels represent pre-incubation of the antibody with immunogenic peptide.
D and F: Immunohistochemical analysis of Cx45 expression in caudal artery (D) and heart tissue (F). Sparse Cx45 expression could be detected in CA and heart (adapted from Rummery, NM et al 2002, Supplementary Figure VII. and Figure 2).

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Species Reactivity

Human

Applications

Host Species

Sheep

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Data Sheet

SKU: S-061-100

Volume/Size: 100 µL

Variant

Price:

$337.00

Quantity:

Product Details

Target

Connexin-45 (Cx45)

Scientific Background

Connexin-45 is a component of gap junctions, which are composed of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low molecular weight diffuse from one cell to a neighboring cell. SUBUNIT: A connexon is composed of a hexamer of connexins. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. SIMILARITY: Belongs to the connexin family. Alpha-type (group II) subfamily. Alternatively spliced isoforms have been described.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

Host Species

Sheep

Molecular Weight

45 kDa (See application details.)

Immunogen

A synthetic peptide (QAYSHQN NPHGPRE) as part of human Connexin-45 protein (aa: 354-367) conjugated to diphtheria toxoid has been used as the immunogen.

Immunogen Species

Human

Species Reactivity

Human

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Dilution Ranges

IHC: 1:100-1:250

Application Details

Immunohistochemistry: Antibody detects sparse Cxn45-IR in caudal artery and heart tissue (see Rummery, NM et al 2002 for more staining specifics). In the original work the specificity of the antibody was shown by incubation either without primary antibody or with primary antibody that had previously been pre-incubated for 1 hour at room temperature with 10-fold excess by weight of the peptide against which the antibody was raised.

Western Blot: Antibody is not recommended for Western blotting, however, it does react in Westerns with Cxn 45 specific material. The authors report that the antibody develops numerous bands in Westerns blots, only some of which are removed upon peptide treatment (see Rummery, NM et al 2002). The Cx45/354 antibody revealed the presence of a specific 45-kDa band in all tissues tested, although it was very weak in the arteries. A higher molecular weight band, which was blocked by peptide, was also seen in the brain (see Rummery, NM et al 2002, online Figure VIIB, -/+ peptide).

Conjugate

Unconjugated

Specificity

Western blotting of cell membranes of COS cells transiently transfected with cDNA encoding Connexin-45 shows a singular band of molecular weight 47 kDa which is abolished by preincubation with the immunising peptide. No such band is detected in Western blots of membranes from non-transfected cells or cells transfected with cDNA encoding Connexin-37 or Connexin-40. This antiserum reacts to human Connexin-45.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Gap junction gamma-1 protein; Gap junction alpha-7 protein; Cx45; GJC1; GJA7

UniProt ID

P36383

Shipping

25°C (ambient)

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Anti-Connexin-45 (Cx45) Antibody

SKU: S-061-100

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