Anti-Connexin 43 (Ser368) Antibody
Our Anti-Connexin 43 (Ser368) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Connexin 43 (Ser368) and is antigen affinity purified from pooled serum. It is great for use in WB.
SKU: p1007-368
Product Details
Connexin 43 (Ser368)
Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at Ser-368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a). Phosphorylation of Ser-368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
GJA1
43 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser368 conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
Bovine, Canine, Chicken, Guinea Pig, Human, Zebrafish
AB_2492064
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
Unconjugated
Specific for endogenous levels of the ~43 kDa connexin-43 protein phosphorylated at Ser368. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Ser368
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Connexin 43 antibody, Connexin-43 antibody, Cx 43 antibody, Cx43 antibody, CXA1_HUMAN antibody, DFNB38 antibody, Gap junction 43 kDa heart protein antibody, Gap junction alpha-1 protein antibody, Gap junction protein alpha 1 43kDa (connexin 43) antibody, Gap junction protein alpha 1 43kDa antibody, Gap junction protein alpha like antibody, GJA 1 antibody, Gja1 antibody, GJAL antibody, ODD antibody, ODDD antibody, ODOD antibody, SDTY3 antibody
UniProt (Human): P17302
UniProt (Immunogen Species): P08050
UniProt (Immunogen Species): P08050
24392
Product Specific Protocols
- Western Blot Protocol: Download
General References
- Aronica E, Gorter JA, Jansen GH, Leenstra S, Yankaya B, Troost D (2001) Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex. Acta Neuropathol (Berl) 101:449-459. PMID: 11484816
- Bao X, Altenberg GA, Reuss L (2004a) Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation. Am J Physiol Cell Physiol 286:C647-C654. PMID: 14602580
- Bao X, Reuss L, Altenberg GA (2004b) Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368. J Biol Chem 279:20058-20066. PMID: 14973142
- Yogo K, Ogawa T, Akiyama M, Ishida N, Takeya T (2002) Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells. FEBS Lett 531:132-136. PMID: 12417300