Anti-Clcn3 Antibody (N258/5)

Name: Anti-Clcn3 Antibody
Brand: NeuroMab
SKU: 75-259
Price: 319.0 USD
Availability: InStock

Our Anti-Clcn3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N258/5. It is KO validated, detects human, mouse, and rat Clcn3, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunoblot versus crude membranes from adult rat brain (RBM) and WT and Clcn3 KO mouse brains probed with N258/5 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Fred Lamb (University of Iowa.

Adult rat brain immunohistochemistry.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

KO Validated

SKU: 75-259

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Clcn3

Target Description

Chloride Voltage-Gated Channel 3, CLCN3 or H(+)/Cl(-) exchange transporter 3 is encoded by the gene CLCN3 and is a member of the voltage-gated chloride channel (ClC) family. CLCN3 is a proton-coupled chloride transporter. It plays a role in acidification of endosomes and synaptic vesicle lumen. CLCN3 implicated in vesicle trafficking, neuronal cell function and short term memory. CLCN3 is expressed in several tissues including brain, aortic and coronary vascular smooth muscle cells, bronchus and colon. Diseases associated with this gene include Dent Disease 1 and Cystic Fibrosis

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N258/5

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

CLCN3

Molecular Weight

90 kDa

Antigen

Synthetic peptide amino acids 98-115 (CKDRERHRRINSKKKESA, cytoplasmic N-terminus) of rat Clcn3 (accession number P51792)

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10671699

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with Clcn4 or Clcn5 (based on KO validation results)

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

H(+)/Cl(-) exchange transporter 3 (Chloride channel protein 3) (ClC-3) (Chloride transporter ClC-3)

UniProt Details

UniProt (Human): P51790
UniProt (Immunogen Species): P51790

Gaurav R1, Bewtra AK1, Agrawal DK1. (2014), 'Novel CLC3 transcript variants in blood eosinophils and increased CLC3 expression in nasal lavage and blood eosinophils of asthmatics..' Immun Inflamm Dis. 10.1002/iid3.36.
Cuddapah VA1, Turner KL, Sontheimer H. (2013), 'Calcium entry via TRPC1 channels activates chloride currents in human glioma cells..' Cell Calcium. 10.1016/j.ceca.2012.11.013.
McMains E1, Krishnan V, Prasad S, Gleason E. (2011), 'Expression and localization of CLC chloride transport proteins in the avian retina..' PLoS One. 10.1371/journal.pone.0017647.

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Anti-Clcn3 Antibody (N258/5)

SKU: 75-259

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