Anti-Choline O-acetyltransferase (ChAT) Antibody

Name: Anti-Choline O-acetyltransferase (ChAT) Antibody
Brand: Biosensis
SKU: R-043-100
Price: 327.0 USD
Availability: InStock

Our Anti-Choline O-acetyltransferase (ChAT) rabbit polyclonal primary antibody detects guinea pig, mouse, pig, rabbit, and rat Choline O-acetyltransferase (ChAT), and is whole serum. It is validated for use in ELISA, ICC, IHC-Frozen, IHC-Paraffin-embedded.

Immunofluorescent staining of rat basal forebrain cholinergic neurons using rabbit anti-ChAT primary and Cy3 secondary antibodies.

Immunohistochemical detection of cholinergic neurons in formalin-fixed floated cryostat sections from the basal forebrain of the rat. Choline acetyltransferase immunoreactivity was detected with the rabbit polyclonal ChAT antiserum (R-043-100; 1:20,000), using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.

Immunofluorescent staining of rat basal forebrain cholinergic neurons using rabbit anti-ChAT primary and Cy3 secondary antibodies.

Click on image to zoom

Species Reactivity

Guinea Pig, Mouse, Pig, Rabbit, Rat

Applications

ELISA, ICC, IHC

Host Species

Rabbit

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Data Sheet

SKU: R-043-100

Volume/Size: 100 µL

Variant

Price:

$327.00

Quantity:

Product Details

Target

Choline O-acetyltransferase (ChAT)

Scientific Background

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

ELISA, ICC, IHC

Host Species

Rabbit

Immunogen

A synthetic peptide (GLFSSYRLPGHTQDTLVAQKSS) as a part of porcine ChAT protein (aa: 167-188) conjugated to KLH

Immunogen Species

Pig

Species Reactivity

Guinea Pig, Mouse, Pig, Rabbit, Rat

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Dilution Ranges

IHC: 1:400-1:2000
ICC: 1:400-1:2000

Application Details

IHC/ICC on 4% PFA or formalin fixed frozen sections; paraffin sections can be more difficult and require more extensive antigen recovery methods. 1:400-1:2000 depending up detection and incubation times
ELISA: direct antigen ELISA
This antiserum will superbly stain both cell bodies and nerve terminal, and works particularly well in enteric and peripheral neurons.

Conjugate

Unconjugated

Specificity

This antiserum stains cholinergic neurons in guinea-pig and rabbit. This antiserum is known to react with ChAT of origin guinea-pig, mouse, rat and rabbit.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Choline O-acetyltransferase; CHOACTase; Choline acetylase

UniProt ID

P13222

Shipping

25°C (ambient)

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Anti-Choline O-acetyltransferase (ChAT) Antibody

SKU: R-043-100

Product Details

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