Anti-CD46 (Extracellular region) Antibody (M037)

Name: Anti-CD46 (Extracellular region) Antibody
Brand: PhosphoSolutions
SKU: CM0371
Price: 379.0 USD
Availability: InStock

Our CD46 (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human CD46 (Extracellular region) and is Protein G purified. It is great for use in WB, ICC, IP.

Western blot of native lysates from NCI-H2052 mesothelial cells (lane 1), SK-MES-1 squamous carcinoma (lane 2), MDA-MB-231 breast carcinoma (lane 3), MeWo melanoma (lane 4), A431 skin adenocarcinoma (lane 5), LNCaP prostate cancer cells (lane 6), and MCF7 breast cancer cells (lane 7). The blot was probed with mouse monoclonal anti-CD46 (CM0371) at 1:1000.

Immunocytochemical labeling of CD46 in aldehyde fixed human NCIH1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD46 (CM0371). The antibody was detected using goat anti-mouse Ig:DyLight® 594.

Representative Standard Curve using mouse monoclonal anti-CD46 (CM0371) for ELISA capture of human recombinant CD46 extracellular region with a His-tag. Captured protein was detected by suitable anti-His-tag antibody followed by appropriate secondary
antibody HRP conjugate.

Western blot of native lysates from NCI-H2052 mesothelial cells (lane 1), SK-MES-1 squamous carcinoma (lane 2), MDA-MB-231 breast carcinoma (lane 3), MeWo melanoma (lane 4), A431 skin adenocarcinoma (lane 5), LNCaP prostate cancer cells (lane 6), and MCF7 breast cancer cells (lane 7). The blot was probed with mouse monoclonal anti-CD46 (CM0371) at 1:1000.

Click on image to zoom

Species Reactivity

Human

Applications

ELISA, ICC, IP, WB

Host Species

Mouse

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Data Sheet

SKU: CM0371

Volume: 100 µL

Variant

Price:

$379.00

Quantity:

Ships: 1-2 business days

Product Details

Target

CD46 (Extracellular region)

Scientific Background

CD46 is a complement regulatory protein that is also called membrane cofactor protein. This protein is a type 1 membrane protein that plays an important inhibitory role in the complement system. CD46 exhibits a cofactor activity that promotes inactivation of C3b and C4b by serum factor 1, thereby protecting host cells from complement-dependent cytotoxicity. CD46 can also function as a receptor for selected bacteria and viruses, and is reportedly required for proper fusion of spermatozoa to the oocyte membrane during fertilization. CD46 is overexpressed in medulloblastoma tumors, and CD46 expression has been linked with poor prognosis in breast cancer. The upregulation of CD46 may protect cancer cells from complement-dependent cytotoxicity to facilitate cancer cell immune evasion.

Format

Protein G Purified

Clonality

Monoclonal

Clone

M037

Isotype

IgG2b

Applications

ELISA, ICC, IP, WB

Host Species

Mouse

Gene Name

CD46

Molecular Weight

45-60

Immunogen

Clone (M037) was generated from a proprietary antigen related to native human CD46 expressed in NCI-H1915 lung cancer cell line.

Immunogen Species

Human

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100
IP: 1:100

Conjugate

Unconjugated

Specificity

Clone M037 mouse monoclonal antibody detects a 45-60 kDa* protein on SDS-PAGE "Native" immunoblots of human A549, MeWo, MDA-MB-231, and MCF7 cells, as well as human lung tissues. This antibody weakly detects denatured CD46. The antibody works for western blot, immunoprecipitation, ELISA capture, and immunocytochemistry.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

AHUS2; Membrane cofactor protein; MIC10; TLX; TRA2.10; CD46 antigen; complement regulatory protein; CD46 molecule; antigen identified by monoclonal TRA-2-10; complement membrane cofactor protein; measles virus receptor; trophoblast leucocyte common antigen; trophoblast leukocyte common antigen; trophoblast-lymphocyte cross-reactive antigen; CD46; MCP

UniProt ID

P15529
UniProt Summary: Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.

NCBI Gene ID

4179

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Anti-CD46 (Extracellular region) Antibody (M037)

SKU: CM0371

Product Details

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