Our CD44 (Hyaluron Binding Region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human CD44 (Hyaluron Binding Region) and is Protein G purified. It is great for use in WB, ICC, IP.
Western blot analysis of denatured (lanes 1 & 3) and native (lanes 2 & 4) human MDA-MB-231 whole cell lysates. The blots were probed with mouse monoclonal anti-CD44 (CM5881) at 1:1000 (lanes 1 & 2) or mouse monoclonal anti-CD44 (CM5911) at 1:1000 (lanes 3 & 4).
Cell surface adhesion protein CD44 is a ubiquitously expressed type I transmembrane protein that has important functions related to cell-cell adhesion and extracellular matrix interactions. The transmembrane protein is post-translationally modified at multiple sites by glycosylation and phosphorylation. CD44 ligands include hyaluronic acid, collagens, laminins, osteopontin, serglycin, and fibronectin. CD44 has been implicated in inflammatory cell functions as well as in tumor growth and metastasis. CD44 is overexpressed in many types of cancer; the interaction between CD44 and HER2 has been linked to an increase in ovarian carcinoma cell growth. CD44 interacts with ezrin, radixin, and moesin to link the actin cytoskeleton to the plasma membrane and the extracellular matrix. These interactions are critical for CD44 function in cell-cell adhesion and cell motility.
Protein G Purified
Monoclonal
M591
IgG1
ICC, IP, WB
Mouse
CD44
80-130
Clone M591 was generated from a proprietary antigen related to the hyaluron binding region of human CD44 from the MDA-MB-231 breast cancer cell line.
Human
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:100
IP: 1:100
Unconjugated
Clone M591 detects 80-130 kDa* bands corresponding to the molecular mass of CD44 on SDS-PAGE immunoblots of denatured and native MDA-MB-231 cell lysates. The antibody also detects the denatured and native form of a recombinant human CD44 protein that includes only the hyaluron binding region. Clone M591 can be used in multiple applications including western blot, immunocytochemical labeling, ELISA, and immunoprecipitation, as well as for detecting CD44 in live, unfixed cells.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
