Anti-CD44 (Extracellular region) Antibody (M010)

Name: Anti-CD44 (Extracellular region) Antibody
Brand: PhosphoSolutions
SKU: CM0101
Price: 379.0 USD
Availability: InStock

Our CD44 (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human CD44 (Extracellular region) and is Protein G purified. It is great for use in WB, ICC, IP.

Immunocytochemical labeling of CD44 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M010). The antibody was detected using goat anti-mouse DyLight® 594.

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Species Reactivity

Human

Applications

ELISA, ICC, IP, WB

Host Species

Mouse

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Data Sheet

SKU: CM0101

Volume: 100 µL

Variant

Price:

$379.00

Quantity:

Ships: 1-2 business days

Product Details

Target

CD44 (Extracellular region)

Scientific Background

Cell surface adhesion protein CD44 is a ubiquitously expressed type I transmembrane protein that has important functions related to cell-cell adhesion and extracellular matrix interactions. The transmembrane protein is post-translationally modified at multiple sites by glycosylation and phosphorylation. CD44 ligands include hyaluronic acid, collagens, laminins, osteopontin, serglycin, and fibronectin. CD44 has been implicated in inflammatory cell functions as well as in tumor growth and metastasis. CD44 is overexpressed in many types of cancer; the interaction between CD44 and HER2 has been linked to an increase in ovarian carcinoma cell growth. CD44 interacts with ezrin, radixin, and moesin to link the actin cytoskeleton to the plasma membrane and the extracellular matrix. These interactions are critical for CD44 function in cell-cell adhesion and cell motility.

Format

Protein G Purified

Clonality

Monoclonal

Clone

M010

Isotype

IgG2b

Applications

ELISA, ICC, IP, WB

Host Species

Mouse

Gene Name

CD44

Molecular Weight

80-130

Immunogen

Clone M010 was generated from a proprietary antigen related to the extracellular region of human CD44.from the A431 epidermoid carcinoma cell line.

Immunogen Species

Human

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100
IP: 1:50

Conjugate

Unconjugated

Specificity

Clone M010 detects 80-130 kDa* bands corresponding to the molecular mass of CD44 on SDS-PAGE immunoblots of native and denaturing human MDA-MB-231, A549, and A431 cell lysates. The antibody can be used for multiple applications including ELISA, western blot, immunocytochemical labeling and immunoprecipitation. In addition, the antibody labels live unfixed A549 and MeWo cells, so the antibody may be useful for isolating CD44+ cells and for CD44 functional assays.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

CDW44; Epican; ECMR-III; HCELL; HUTCH-I; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CD44 antigen; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; extracellular matrix receptor III; hematopoietic cell E- and L-selectin ligand; heparan sulfate proteoglycan; homing function and Indian blood group system; hyaluronate receptor; phagocytic glycoprotein 1; soluble CD44; PGP-1; MIC4 Heparan sulfate proteoglycan HUTCH-I GP90; CD44

UniProt ID

P16070
UniProt Summary: Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment. Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection. Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases. Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion. Upon interaction with LGALS9 ligand, activates downstream signaling components including LCK, ERK and MAPK to promotes NK cell activation.

NCBI Gene ID

960

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Anti-CD44 (Extracellular region) Antibody (M010)

SKU: CM0101

Product Details

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