Anti-Caveolin-1 (Tyr-14), Phosphospecific Antibody (M283)

Name: Anti-Caveolin-1 (Tyr-14), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: CM2831
Price: 360.0 USD
Availability: InStock

Our Caveolin-1 (Tyr-14) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Caveolin-1 (Tyr-14) and is Protein A purified. It is great for use in WB, ICC.

Immunocytochemical labeling of caveolin-1 phosphorylation in rabbit spleen fibroblasts. The cells were treated with pervanadate (1 mM) for 30 min, then fixed with paraformaldehyde and labeled with rabbit polyclonal Caveolin-1 (N-terminal region) and mouse monoclonal Caveolin-1 (Tyr-14) antibodies. The antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Mouse

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SKU: CM2831

Volume: 100 µL

Variant

Price:

$360.00

Quantity:

Product Details

Target

Caveolin-1 (Tyr-14)

Target Description

Caveolins are the primary structural components of the plasma membrane microdomains, caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified, and each has distinct expression patterns. Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion and apoptosis. Caveolins can interact with various signaling molecules, including G-proteins, receptor tyrosine kinases, PKCs, and Src family kinases. Phosphorylation at Tyr-14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, while phosphorylation at Ser-80 regulates caveolin binding to the ER membrane and entry into the secretory pathway.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M283

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

CAV1

Molecular Weight

Antigen

Clone M283 was generated from a phospho-Caveolin-1 (Tyr-14) synthetic peptide corresponding to amino acids surrounding tyrosine 14 in human Caveolin-1. This sequence has significant homology to the conserved site in rat and mouse Caveolin-1.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

This antibody detects a 21 kDa* protein corresponding to the apparent molecular mass of caveolin-1 on SDS-PAGE immunoblots of human A431 cells treated with EGF or pervanadate, and is not observed in untreated cells.

Post Translational Modification Type

GOmAb/Phosphorylated

Post Translational Modification Residue

Tyr-14

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

caveolin1, vip21

UniProt Details

UniProt (Human): Q03135
UniProt (Immunogen Species): Q03135

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Anti-Caveolin-1 (Tyr-14), Phosphospecific Antibody (M283)

SKU: CM2831

Product Details

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