Anti-Cav1.2 Ca2+ Channel Antibody (N263/31)

from NeuroMab Datasheet
22 Citations

Available Conjugates

490
550
594
650

Primary Antibody
Guinea Pig, Human, Mouse, Rabbit, Rat
N263/31
ICC, IHC, WB
Mouse
Purified by Protein A chromatography
Cacna1c Cach2 Cacn2 Cacnl1a1 Cchl1a1
240 kDa

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Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with untagged Cav1.2, Cav1.3 or Kv2.1 plasmids and probed with N263/31 (left) and L57/46 (right) TC supe.
TIRF images (top row), super-resolution GSD localization maps (middle row) and binarized images (bottom row) of immunostained CaV1.2 (cat. 75-257, 1:200) channels and β1ARs in representative fixed adult ventricular myocytes isolated from WT mice under control (A) or ISO-stimulated conditions (B). Merged two-channel image showing relative distributions of CaV1.2 and β1ARs is shown in the third column with the colocalized binarized image. Image from publication CC-BY-4.0. PMID:37438479
Representative maximum intensity projections from WT (black) and NPC1I1061T (red) mouse cerebellar sagittal sections co-immunolabeled for Calbindin(cat. 75-448, 2ug/ml) , CaV1.2 (cat. 75-257, 5ug/ml) and KV2.1 (cat. 75-014, 10ug/ml). Image from publication CC-BY-4.0. PMID:37507375
Representative super-resolution TIRF images of CTL (black) and U18-treated (red) neurons co-immunolabeled for CaV1.2 (cat. 75-257, 5ug/ml) and KV2.1 (cat. 75-014, 10ug/ml). Image from publication CC-BY-4.0. PMID:37507375
Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with untagged Cav1.2, Cav1.3 or Kv2.1 plasmids and probed with N263/31 (left) and L57/46 (right) TC supe.
Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with untagged Cav1.2, Cav1.3 or Kv2.1 plasmids and probed with N263/31 (left) and L57/46 (right) TC supe.
TIRF images (top row), super-resolution GSD localization maps (middle row) and binarized images (bottom row) of immunostained CaV1.2 (cat. 75-257, 1:200) channels and β1ARs in representative fixed adult ventricular myocytes isolated from WT mice under control (A) or ISO-stimulated conditions (B). Merged two-channel image showing relative distributions of CaV1.2 and β1ARs is shown in the third column with the colocalized binarized image. Image from publication CC-BY-4.0. PMID:37438479
Representative maximum intensity projections from WT (black) and NPC1I1061T (red) mouse cerebellar sagittal sections co-immunolabeled for Calbindin(cat. 75-448, 2ug/ml) , CaV1.2 (cat. 75-257, 5ug/ml) and KV2.1 (cat. 75-014, 10ug/ml). Image from publication CC-BY-4.0. PMID:37507375
Representative super-resolution TIRF images of CTL (black) and U18-treated (red) neurons co-immunolabeled for CaV1.2 (cat. 75-257, 5ug/ml) and KV2.1 (cat. 75-014, 10ug/ml). Image from publication CC-BY-4.0. PMID:37507375

Anti-Cav1.2 Ca2+ Channel Antibody
SKU: 75-257

Volume: 100 µL
1-2 business days
Price:
$308

Bulk Order Anti-Cav1.2 Ca2+ Channel Antibody

Product Specific References for Applications and Species

Fluorescence Resonance Energy Transfer: Rat
PMID Dilution Publication
270766161:100Bannister, J.P., et al. 2016. Rab25 influences functional Cav1.2 channel surface expression in arterial smooth muscle cells. American Journal of Physiology. Cell Physiology, C885-C895.
Immunocytochemistry: Guinea Pig
PMID Dilution Publication
314034021:200Morgenstern, T.J., et al. 2019. A potent voltage-gated calcium channel inhibitor engineered from a nanobody targeted to auxiliary CaVβ subunits. Elife, e49253.
Immunocytochemistry: Mouse
PMID Dilution Publication
374384791:100Cserne Szappanos, H., et al. 2023. Cytoskeletal Disarray Increases Arrhythmogenic Vulnerability During Sympathetic Stimulation in a Model of Hypertrophic Cardiomyopathy. Scientific Reports, 11296.
3308233910ug/mlPrada, M.P., et al. 2020. AKAP5 complex facilitates purinergic modulation of vascular L-type Ca2+ channel CaV1.2. Nature Communications, 5303.
316638501:5 (supe)Vierra, N.C., et al. 2019. Kv2. 1 mediates spatial and functional coupling of L-type calcium channels and ryanodine receptors in mammalian neurons. Elife, e49953.
Immunocytochemistry: Rat
PMID Dilution Publication
344319811:100Isensee, J., et al. 2021. Depolarization induces nociceptor sensitization by CaV1.2-mediated PKA-II activation. Journal of Cell Biology, .
27364017not listedEichel, C.A., et al. 2016. Lateral Membrane-Specific MAGUK CASK Down-Regulates NaV1.5 Channel in Cardiac Myocytes. Circulation Research, 544-56.
Immunohistochemistry: Human
PMID Dilution Publication
281194641:1000Nystoriak, M.A., et al. 2017. Ser1928 phosphorylation by PKA stimulates the L-type Ca2+ channel CaV1.2 and vasoconstriction during acute hyperglycemia and diabetes. Scientific Signaling, eaaf9647.
Immunohistochemistry: Mouse
PMID Dilution Publication
375073755ug/mlCasas, M., et al. 2023. NPC1-dependent alterations in KV2.1–CaV1.2 nanodomains drive neuronal death in models of Niemann-Pick Type C disease. Nature Communications, 4553.
Immunoprecipitation: Rat
PMID Dilution Publication
246813471:500Liu, W., et al. 2014. KCNE2 modulates cardiac L-type Ca(2+) channel.. Journal of Molecular Cellular Cardiology, 208-218.
PLA: Human
PMID Dilution Publication
281194641:1000Nystoriak, M.A., et al. 2017. Ser1928 phosphorylation by PKA stimulates the L-type Ca2+ channel CaV1.2 and vasoconstriction during acute hyperglycemia and diabetes. Scientific Signaling, eaaf9647.
STED: Rabbit
PMID Dilution Publication
29549309not listedZhang, X.D., et al. 2018. Coupling of SK channels, L-type Ca2+ channels, and ryanodine receptors in cardiomyocytes. Scientific Reports, 4670.
Western Blot: Guinea Pig
PMID Dilution Publication
267644821:500Nassal, D.M., et al. 2016. Myocardial KChIP2 expression in guinea pig resolves an expanded electrophysiologic role. PLoS One, e0146561.
Western Blot: Human
PMID Dilution Publication
244016931:200Duan, L., et al. 2014. Stem cell derived basal forebrain cholinergic neurons from Alzheimer''s disease patients are more susceptible to cell death.. Molecular Neurdegeneration, 3.
238580111:250Narayanan, D., et al. 2013. Smooth muscle cell transient receptor potential polycystin-2 (TRPP2) channels contribute to the myogenic response in cerebral arteries.. Journal of Physiology, 5031-5046.
Western Blot: Mouse
PMID Dilution Publication
32442021not listedGupte, R., et al. 2020. Glucose-6-phosphate dehydrogenase increases Ca2+ currents by interacting with Cav1.2 and reducing intrinsic inactivation of the L-type calcium channel. American Journal of Physiology. Heart and Circulatory Physiology, H144-H158.
Western Blot: Rat
PMID Dilution Publication
334532401:1000Nasu, F., et al. 2021. Azelnidipine treatment reduces the expression of Cav1.2 protein. Life Sciences, 119043.
31337710not listedJiang, M., et al. 2019. S-Palmitoylation of junctophilin-2 is critical for its role in tethering the sarcoplasmic reticulum to the plasma membrane. Journal of Biological Chemistry, 13487-13501.
256103721:1000Perissinotti, P.P., et al. 2015. Calcium current homeostasis and synaptic deficits in hippocampal neurons from Kelch-like 1 knockout mice.. Frontiers in Cellular Neuroscience, 444.
247039041:1000Perissinotti, P.P., et al. 2014. Down-regulation of endogenous KLHL1 decreases voltage-gated calcium current density.. Cell Calcium, 269-280.
238580111:250Narayanan, D., et al. 2013. Smooth muscle cell transient receptor potential polycystin-2 (TRPP2) channels contribute to the myogenic response in cerebral arteries.. Journal of Physiology, 5031-5046.
23568894not listedBannister, J.P., et al. 2013. The voltage-dependent L-type Ca2+ (CaV1.2) channel C-terminus fragment is a bi-modal vasodilator. Journal of Physiology, 2987-2998.

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