Anti-Caspase-8 (C-terminal region) Antibody (M382)

Name: Anti-Caspase-8 (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: CM3821
Price: 292.0 USD
Availability: InStock

Our Caspase-8 (C-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Caspase-8 (C-terminal region) and is Protein A purified. It is great for use in WB.

Western blot analysis of Caspase expression in human Jurkat cells. The blot was probed with anti-Caspase-3 at 1:500 (lane 1) and 1:1000 (lane 2), anti-Caspase-6 at 1:250 (lane 3) and 1:500 (lane 4), anti-Caspase-7 at 1:500 (lane 5) and 1:1000 (lane 6), as well as anti-Caspase-8 at 1:250 (lane 7) and 1:500 (lane 8).

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Species Reactivity

Human

Applications

ELISA, WB

Host Species

Mouse

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Data Sheet

SKU: CM3821

Volume: 100 µL

Variant

Price:

$292.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Caspase-8 (C-terminal region)

Scientific Background

The caspases are a group of cysteine enzymes, which cleave proteins in response to intrinsic and extrinsic pathways that cause apoptotic cell death. The caspases can be grouped into two subgroups based on their roles in apoptosis. Initiator caspases (caspases 2, 8, 9, and 10) are activated through the apoptosis-signaling pathways and activate the effector caspases (caspases 3, 6, and 7) which carry out apoptosis. Caspase cascades are initiated through assembly of multiprotein complexes that trigger activation of the initiator caspases, which are then released and are able to activate the downstream effector caspases.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M382

Isotype

IgG2a

Applications

ELISA, WB

Host Species

Mouse

Gene Name

CASP8

Molecular Weight

Immunogen

Clone (M382) was generated from a recombinant protein containing amino acid residues in the C-terminal region of human Caspase-8.

Immunogen Species

Human

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500

Conjugate

Unconjugated

Specificity

The antibody detects a 55 kDa* protein corresponding to the molecular weight of Caspase-8 on SDS-PAGE immunoblots of human Jurkat, K562, and A431 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

ALPS2B; Caspase-8; Casp-8; FLICE; MACH; MCH5; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; ICE-like apoptotic protease 5; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MORT1-associated ced-3 homolog; apoptotic cysteine protease; apoptotic protease Mch-5; caspase 8; apoptosis-related cysteine peptidase; apoptosis-related cysteine protease; ced3; NEDD2-like protein,p15; p10

UniProt ID

Q14790
UniProt Summary: Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood. Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for FAS/CD95-mediated and TNFRSF1A-induced cell death. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. Binding to the adapter molecule FADD recruits it to either receptor FAS/TNFRSF6 or TNFRSF1A. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Also cleaves and activates BID, thereby promoting cytochrome C release from mitochrondria. In addition to extrinsic apoptosis, also acts as a negative regulator of necroptosis: acts by cleaving RIPK1 at 'Asp-324', which is crucial to inhibit RIPK1 kinase activity, limiting TNF-induced apoptosis, necroptosis and inflammatory response. Also able to initiate pyroptosis by mediating cleavage and activation of gasdermin-C and -D (GSDMC and GSDMD, respectively): gasdermin cleavage promotes release of the N-terminal moiety that binds to membranes and forms pores, triggering pyroptosis. Initiates pyroptosis following inactivation of MAP3K7/TAK1. Also acts as a regulator of innate immunity by mediating cleavage and inactivation of N4BP1 downstream of TLR3 or TLR4, thereby promoting cytokine production. May participate in the Granzyme B (GZMB) cell death pathways. Cleaves PARP1 and PARP2. Independent of its protease activity, promotes cell migration following phosphorylation at Tyr-380.

NCBI Gene ID

841

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Anti-Caspase-8 (C-terminal region) Antibody (M382)

SKU: CM3821

Product Details

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