Our Anti-Calretinin primary antibody from PhosphoSolutions is sheep polyclonal. It detects rat Calretinin and is Protein G purified. It is great for use in WB, IHC.
Western blot of rat cerebellar lysate showing specific immunolabeling of the ~29 kDa calretinin protein.
Calretinin is a calcium-binding protein involved in calcium signaling. It is abundantly expressed throughout the central nervous system, retina and many other tissues. Studies have shown that calretinin may play a role in neuroprotection by buffering intracellular calcium (Lukas and Jones 1994, Weisenhorn et al., 1996). Additionally, calretinin selectively stains mesothelial cells thus making it an important marker for differentiating malignant mesothelioma from carcinomas (Attanoos et al., 2000).
Protein G Purified
Polyclonal
IgG
IHC, WB
Sheep
CALB2
29 kDa
Purified guinea pig calretinin.
Rat
Human, Mouse, Non-Human Primate
AB_2492050
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Protein G purified sheep serum.
10 mM Tris, 100 mM NaCl
WB: 1:1000
WB Brain: 1:1000
IHC: 1:500
Unconjugated
Specific for endogenous levels of the ~29 kDa calretinin protein in Western blots of rat brain lysate. The antibody also works well for immunohistochemistry on formalin or paraformaldehyde perfused rat brain tissue sections.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Attanoos, R.L., Dojcinov, S.D., Webb, R. and Gibbs, A.R., 2000. Anti‐mesothelial markers in sarcomatoid mesothelioma and other spindle cell neoplasms. Histopathology, 37(3), pp.224-231. PMID: 10971698
Isaacs, K.R., Winsky, L., Strauss, K.I. and Jacobowitz, D.M., 1995. Quadruple colocalization of calretinin, calcitonin gene-related peptide, vasoactive intestinal peptide, and substance P in fibers within the villi of the rat intestine. Cell and tissue research, 280(3), pp.639-651. PMID: 7541720
Lukas, W. and Jones, K.A., 1994. Cortical neurons containing calretinin are selectively resistant to calcium overload and excitotoxicity in vitro. Neuroscience, 61(2), pp.307-316. PMID: 7969911
Weisenhorn, D.V., Weruaga-Prieto, E. and Celio, M.R., 1996. Calretinin-immunoreactivity in organotypic cultures of the rat cerebral cortex: effects of serum deprivation. Experimental brain research, 108(1), pp.101-112. PMID: 8721158
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