Anti-Calretinin Antibody

Name: Anti-Calretinin Antibody
Brand: PhosphoSolutions
SKU: 303-CAL
Price: 456.0 USD
Availability: InStock

Our Anti-Calretinin primary antibody from PhosphoSolutions is sheep polyclonal. It detects rat Calretinin and is Protein G purified. It is great for use in WB, IHC.

Western blot of rat cerebellar lysate showing specific immunolabeling of the ~29 kDa calretinin protein.

Immunostaining of rat cerebellum showing fluorescence of calretinin (Cat. 303-CAL, green, 1:500) containing cells.

Western blot of rat cerebellar lysate showing specific immunolabeling of the ~29 kDa calretinin protein.

Click on image to zoom

Species Reactivity

Rat

Applications

ELISA, IHC, WB

Host Species

Sheep

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Data Sheet

Trial Size

SKU: 303-CAL

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Calretinin

Scientific Background

Calretinin is a calcium-binding protein involved in calcium signaling. It is abundantly expressed throughout the central nervous system, retina and many other tissues. Studies have shown that calretinin may play a role in neuroprotection by buffering intracellular calcium (Lukas and Jones 1994, Weisenhorn et al., 1996). Additionally, calretinin selectively stains mesothelial cells thus making it an important marker for differentiating malignant mesothelioma from carcinomas (Attanoos et al., 2000).

Format

Protein G Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, IHC, WB

Host Species

Sheep

Gene Name

CALB2

Molecular Weight

29 kDa

Immunogen

Purified guinea pig calretinin.

Immunogen Species

Guinea Pig

Species Reactivity

Rat

Expected Reactivity

Human, Mouse, Non-Human Primate

Antibody Registry ID

AB_2492050

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Protein G purified sheep serum.

Buffer

10 mM Tris, 100 mM NaCl

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~29 kDa calretinin protein in Western blots of rat brain lysate. The antibody also works well for immunohistochemistry on formalin or paraformaldehyde perfused rat brain tissue sections.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

29 kDa calbindin; CAB 29; CAB29; CAL 2; CAL2; CALB 2; CALB2; CALB2_HUMAN; Calbindin 2 29kDa; Calbindin 2; Calbindin D29K; Calbindin2; Calretinin; CR

UniProt ID

P47728
UniProt Summary: Calcium-binding protein involved in calcium homeostasis and signal transduction. It plays a critical role in buffering intracellular calcium levels and modulating calcium-dependent signaling pathways (PubMed:2001709). Predominantly expressed in specific neuronal populations, influences synaptic plasticity and neuronal excitability, contributing to learning and memory (By similarity). During embryonic development, it facilitates neuronal differentiation and maturation (By similarity). {ECO:0000250|UniProtKB:P47728, ECO:0000250|UniProtKB:Q08331, ECO:0000269|PubMed:2001709}.

NCBI Gene ID

117059

Shipping

Blue Ice

Western Blot Protocol: Download

General References

Attanoos, R.L., Dojcinov, S.D., Webb, R. and Gibbs, A.R., 2000. Anti‐mesothelial markers in sarcomatoid mesothelioma and other spindle cell neoplasms. Histopathology, 37(3), pp.224-231. PMID: 10971698
Isaacs, K.R., Winsky, L., Strauss, K.I. and Jacobowitz, D.M., 1995. Quadruple colocalization of calretinin, calcitonin gene-related peptide, vasoactive intestinal peptide, and substance P in fibers within the villi of the rat intestine. Cell and tissue research, 280(3), pp.639-651. PMID: 7541720
Lukas, W. and Jones, K.A., 1994. Cortical neurons containing calretinin are selectively resistant to calcium overload and excitotoxicity in vitro. Neuroscience, 61(2), pp.307-316. PMID: 7969911
Weisenhorn, D.V., Weruaga-Prieto, E. and Celio, M.R., 1996. Calretinin-immunoreactivity in organotypic cultures of the rat cerebral cortex: effects of serum deprivation. Experimental brain research, 108(1), pp.101-112. PMID: 8721158

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Anti-Calretinin Antibody

SKU: 303-CAL

Product Details

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