Anti-c-Src (Tyr-215) [conserved site], Phosphospecific Antibody

Name: Anti-c-Src (Tyr-215) [conserved site], Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: SP1371
Price: 329.0 USD
Availability: InStock

2 Citations

Our c-Src (Tyr-215) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat c-Src (Tyr-215) and is antigen affinity purified. It is great for use in WB.

Western blot analysis of mouse SYF cells transformed with c-Src then left untreated (lanes 1, 3, & 5) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4, & 6). The blot was probed with anti-c-Src (Tyr-215) (lanes 1 & 2), anti-c-Src (N-terminal region) (lanes 3 & 4), and anti-c-Src (Tyr-530) (lanes 5 & 6).

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

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SKU: SP1371

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Product Details

Target

c-Src (Tyr-215)

Target Description

c-Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. The Src family is composed of nine members in vertebrates, including c-Src, Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck, and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an N-terminal cell membrane anchor followed by SH3 and SH2 domains. The activity of c-Src is regulated by tyrosine phosphorylation at multiple sites. Tyrosine 418 is autophosphorylated following c-Src activation. Tyrosine 215 in the SH2 domain of c-Src is phosphorylated following growth factor receptor activation. Both Tyr-215 and Tyr-418 phosphorylation increases tyrosine kinase activity, while phosphorylation of Tyr-530 downregulates c-Src kinase activity. Thus, tyrosine phosphorylation of c-Src is critical for regulating its kinase activity.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

SRC

Molecular Weight

Antigen

c-Src (Tyr-215) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 215 of human c-Src. This sequence has homology to the conserved site in other Src family members, such as hck, fyn, csk, and yes.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-c-Src (Tyr-215) peptide (without carrier). The antibody detects a 60 kDa* protein on SDS-PAGE immunoblots of mouse SYF cells transformed with c-Src and human A431 cells that have been treated with pervanadate.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-215

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Src

UniProt Details

UniProt (Human): P12931
UniProt (Immunogen Species): P12931

Product Specific References for Applications and Species

Western Blot: Mouse
Western Blot: Rat

Western Blot: mouse
PMID	Dilution	Publication
21915273	not listed	Lindquist, S, et al. 2011. Phosphoprotein Associated with Glycosphingolipid-Enriched Microdomains Differentially Modulates Src Kinase Activity in Brain Maturation. PLoS One, e23978.

Western Blot: Rat
PMID	Dilution	Publication
26979522	not listed	Pentassuglia, L, et al. 2016. Neuregulin-1β promotes glucose uptake via PI3K/Akt in neonatal rat cardiomyocytes. Americal Journal of Physiology. Endocrinology and Metabolism, E782-794.

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Anti-c-Src (Tyr-215) [conserved site], Phosphospecific Antibody

SKU: SP1371

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-c-Src (Tyr-215) [conserved site], Phosphospecific Antibody

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