Anti-C-Raf (C-terminus) Antibody

Name: Anti-C-Raf (C-terminus) Antibody
Brand: PhosphoSolutions
SKU: RP2071
Price: 292.0 USD
Availability: InStock

Our C-Raf (C-terminus) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat C-Raf (C-terminus) and is antigen affinity purified. It is great for use in WB.

Western blot of GST fusion protein containing human C-Raf. The blot was probed with polyclonal anti-C-Raf (C-terminus) antibody in the presence (lanes 2-4) or absence (lane 1) of C-Raf (C-terminus) blocking peptide (lane 2), C-Raf (Ser-471) peptide (lane 3), or unrelated peptide (lane 4).

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, WB

Host Species

Rabbit

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Data Sheet

SKU: RP2071

Volume: 100 µL

Variant

Price:

$292.00

Quantity:

Ships: 1-2 business days

Product Details

Target

C-Raf (C-terminus)

Scientific Background

The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, B-Raf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (S259)/B-Raf(S365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and multiple catalytic domain sites. EGF receptor activation leads to phosphorylation of Ser-471, which is critical for C-Raf kinase activity and is required for interaction with MEK. In B-Raf, the corresponding conserved site is Ser-578, and mutation of this residue to alanine produces an inactivate kinase. Thus, this Raf phosphorylation site may be critical for kinase activity and may be important for MEK binding and activation

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB

Host Species

Rabbit

Gene Name

RAF1

Molecular Weight

Immunogen

C-Raf synthetic peptide (coupled to KLH) corresponds to amino acids from the C-terminus of human C-Raf. This sequence is conserved in C-Rafs from many species, and is not found in A-Raf or B-Raf.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody detects C-Raf in western blots of a purified GST-fusion protein containing human C-Raf.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

CMD1NN; Raf1; NS5; Raf-1; c-Raf; C-Raf proto-oncogene; serine/threonine kinase; Oncogene RAF1; raf proto-oncogene serine/threonine protein kinase; proto-oncogene c-RAF; RAF proto-oncogene serine/threonine-protein kinase; v-raf-1 murine leukemia viral oncogene homolog 1; CRaf

UniProt ID

P04049
UniProt Summary: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

NCBI Gene ID

5894

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Anti-C-Raf (C-terminus) Antibody

SKU: RP2071

Product Details

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