Anti-c-FOS Antibody (2H2)

5 Citations

Our Anti-c-FOS primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, human, mouse, and rat c-FOS and is Protein G purified. It is great for use in WB, IHC, ICC.

Immunofluorescence of serum starved, FBS stimulated HeLa cells showing nuclear labeling of activated cells with Anti- c-FOS (cat. 309-cFOS, 1:1000, green) while Anti-Vimentin (cat. 2105-VIM, 1:500, red) labels the cytoplasmic intermediate filament, and nuclear staining with DAPI (blue).
Immunofluorescence of a section of rat hippocampus showing neurons labeled with c-FOS (cat. 309-cFOS, 1:1000, red) counterstained with FOX3(NeuN) (green), and nuclear staining with DAPI. When both c-FOS and FOX3 are expressed the label appears orange.
Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa c-FOS protein.
Immunostaining of mouse brain labeling c-Fos protein in green(cat. 309-cFOS, 1:1000) and DNA with DAPI. Photo courtesy of Adam Almeida, University of Colorado.
Representative images of CA1 region of mouse hippocampus. White arrows indicate cFos+ cells (cat. 309-cFOS), yellow arrows indicate 53BP1 + cells. Nuclei stained with DAPI (blue). Right: zoomed images of the white boxes. Image from publication CC-BY-4.0. PMID: 36732588
Immunolabeling of a section of rat brain that specifically labels c-FOS protein(cat. 309-cFOS, green, 1:1000). Frozen sections were fixed with 4%PFA for 24 hours. Before performing free floating immunohistochemistry, sections underwent antigen retrieval with sodium citrate (pH6.0) for 30 minutes at 80˚C. Image kindly provided by Angela Gonzalez, Washington State University, Vancouver.
(A, B) confocal microscope images showing PV, WFA, and c-Fos (cat. 309-cFOS) staining after vehicle or ABC treatment in the prelimbic PFC after vehicle or ABC 31 days later. White arrows indicate triple-labelled cells. Note faint staining of PNNs after ABC treatment. Image from publication CC-BY-4.0. PMID: 37855072.
Immunofluorescence of serum starved, FBS stimulated HeLa cells showing nuclear labeling of activated cells with Anti- c-FOS (cat. 309-cFOS, 1:1000, green) while Anti-Vimentin (cat. 2105-VIM, 1:500, red) labels the cytoplasmic intermediate filament, and nuclear staining with DAPI (blue).
Immunofluorescence of serum starved, FBS stimulated HeLa cells showing nuclear labeling of activated cells with Anti- c-FOS (cat. 309-cFOS, 1:1000, green) while Anti-Vimentin (cat. 2105-VIM, 1:500, red) labels the cytoplasmic intermediate filament, and nuclear staining with DAPI (blue).
Immunofluorescence of a section of rat hippocampus showing neurons labeled with c-FOS (cat. 309-cFOS, 1:1000, red) counterstained with FOX3(NeuN) (green), and nuclear staining with DAPI. When both c-FOS and FOX3 are expressed the label appears orange.
Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa c-FOS protein.
Immunostaining of mouse brain labeling c-Fos protein in green(cat. 309-cFOS, 1:1000) and DNA with DAPI. Photo courtesy of Adam Almeida, University of Colorado.
Representative images of CA1 region of mouse hippocampus. White arrows indicate cFos+ cells (cat. 309-cFOS), yellow arrows indicate 53BP1 + cells. Nuclei stained with DAPI (blue). Right: zoomed images of the white boxes. Image from publication CC-BY-4.0. PMID: 36732588
Immunolabeling of a section of rat brain that specifically labels c-FOS protein(cat. 309-cFOS, green, 1:1000). Frozen sections were fixed with 4%PFA for 24 hours. Before performing free floating immunohistochemistry, sections underwent antigen retrieval with sodium citrate (pH6.0) for 30 minutes at 80˚C. Image kindly provided by Angela Gonzalez, Washington State University, Vancouver.
(A, B) confocal microscope images showing PV, WFA, and c-Fos (cat. 309-cFOS) staining after vehicle or ABC treatment in the prelimbic PFC after vehicle or ABC 31 days later. White arrows indicate triple-labelled cells. Note faint staining of PNNs after ABC treatment. Image from publication CC-BY-4.0. PMID: 37855072.

Bovine, Human, Mouse, Rat
ICC, IHC, WB
Mouse

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SKU: 309-cFOS

Volume: 100 µL
Price:
$430.00

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
PMID Dilution Publication
36732588not listedBoutros, S.W., et al. 2023. Age, Sex, and Apolipoprotein E Isoform Alter Contextual Fear Learning, Neuronal Activation, and Baseline DNA Damage in the Hippocampus. Molecular Psychiatry, .
35468398 1:300 He, Z., et al. 2022. Genetic Deletion of TrpV1 and TrpA1 Does not Alter Avoidance of or Patterns of Brainstem Activation to Citric Acid in Mice. Chemical Senses, 45(7), pp.573-579.
32572463 1:1000 Yu, T., et al. 2020. Genetic Deletion of TrpV1 and TrpA1 Does not Alter Avoidance of or Patterns of Brainstem Activation to Citric Acid in Mice. Chemical Senses, 45(7), pp.573-579.
Immunohistochemistry: Rat
PMID Dilution Publication
367781321:5000Maletta, T., et al. 2023. Preadolescent Exposure to a Sexually Mature, Unrelated Male Rat Reduces Postadolescent Social Recognition Memory and CA2 c-Fos Labeling. Frontiers in Behavioral Neuroscience, 1104866.
34650409 1:800 Ferraro, S., et al. 2021. In utero Exposure to Valproic-Acid Alters Circadian Organisation and Clock-Gene Expression: Implications for Autism Spectrum Disorders. Frontiers in Behavioral Neuroscience, 15.

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