Anti-Brain-derived neurotrophic factor (BDNF) Antibody

Name: Anti-Brain-derived neurotrophic factor (BDNF) Antibody
Brand: Biosensis
SKU: S-015-500
Price: 327.0 USD
Availability: InStock

Our Anti-Brain-derived neurotrophic factor (BDNF) sheep polyclonal primary antibody detects human and rat Brain-derived neurotrophic factor (BDNF), and is IgG. It is validated for use in ELISA, IHC-Frozen, Neutralization, WB.

Species Reactivity

Human, Rat

Applications

ELISA, IHC, Neutralize, WB

Host Species

Sheep

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Data Sheet

SKU: S-015-500

Volume/Size: 500 µg

Variant

Price:

$327.00

Quantity:

Product Details

Target

Brain-derived neurotrophic factor (BDNF)

Scientific Background

BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.

Format

IgG

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, IHC, Neutralize, WB

Host Species

Sheep

Immunogen

Recombinant human BDNF

Immunogen Species

Human

Species Reactivity

Human, Rat

Storage

Spin vial briefly before opening. Reconstitute in 500 µL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Production Notes

Protein G purified IgG

Buffer

Lyophilized from a solution containing PBS pH 7.4

Dilution Ranges

IHC: 1-10 µg/mL

Application Details

Recommended to be used at an amount of 1-10 µg/mL for immunohistochemistry on Zamboni's fixed, frozen tissue. Not recommended for paraffin embedded tissues. Primary use is for biological activity in vitro and in vivo. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antibody does not react to BDNF in Western blot, thus Western blot is not a recommended application.

Conjugate

Unconjugated

Specificity

A cross reactivity of less than 1% against mouse NGF, recombinant human NT3 or NT4/5 has been shown by one site ELISA. Known to react with BDNF from rat and human.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Brain-derived neurotrophic factor; Abrineurin; proBDNF;

UniProt ID

P23560

Shipping

25°C (ambient)

Technical Note 4 - Acid Extraction of Brain Tissue

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Anti-Brain-derived neurotrophic factor (BDNF) Antibody

SKU: S-015-500

Product Details

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