Anti-Botch Antibody (N116/14)

Name: Anti-Botch Antibody
Brand: NeuroMab
SKU: 75-181
Price: 308.0 USD
Availability: InStock

from NeuroMab Datasheet

2 Citations

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

N116/14

Applications

ICC, WB

Host Species

Mouse

Format

Purified

Gene Name

Chac1 Botch Npg7

Molecular Weight

25 kDa

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Adult rat hippocampus immunohistochemistry.

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Myc- tagged Botch1 and untagged Kv2.1 plasmids and probed with N116/14 (left panel) and K89/34 (right panel) TC supe.

Volume: 100 µL

Variant

Price:

$308

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Product Details

Target

Botch

Target Description

Cation transport regulator-like protein 1, Glutathione-Specific Gamma-Glutamylcyclotransferase 1 or Botch1 is encoded by the gene CHAC1. Chac1 is a member of the gamma-glutamylcyclotransferase family. It is an enzyme that acts to catalyze the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Chac1 is involved in neuronal differentiation by deglycination of the Notch receptor, which prevents receptor maturation and inhibits Notch signaling. CHAC1 is expressed in tissues including brain (forebrain and anterior spinal cord), and heart. Diseases associated with CHAC1 include Ovarian Embryonal Carcinoma and Ovarian Cancer.

Format

Purified

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N116/14

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

Chac1 Botch Npg7

Molecular Weight

25 kDa

Antigen

Fusion protein amino acids 1-222 (full-length) of rat Botch1 (also known as Cation transport regulator-like protein 1 or Chac1, accession number B3STU3.1); Mouse: 96% identity (215/222 amino acids identical); Human: 89% identity (198/222 amino acids identical); Greater than 50% identity with Botch2/Chac2

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2079324

Storage

Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Buffer

10mM Tris, 50mM Sodium Chloride, 0.065 % Sodium Azide pH 7.4

Conjugate

Unconjugated

Specificity

No cross-reactivity against Botch2/Chac2

Quality Control

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from opening

Synonyms

Glutathione-specific gamma-glutamylcyclotransferase 1 (Gamma-GCG 1) (EC 4.3.2.7) (Blocks Notch protein) (Botch) (Cation transport regulator-like protein 1) (Neuroprotective protein 7)

UniProt Details

UniProt (Human): Q9BUX1
UniProt (Immunogen Species): B3STU3

Chi Z, Byrne ST, Dolinko A, Harraz MM, Kim MS, Umanah G, Zhong J, Chen R, Zhang J, Xu J, Chen L, Pandey A, Dawson TM, Dawson VL. (2014), 'Botch is a γ-glutamyl cyclotransferase that deglycinates and antagonizes Notch..' Cell Report. 10.1016/j.celrep.2014.03.048.
Chi Z, Zhang J, Tokunaga A, Harraz MM, Byrne ST, Dolinko A, Xu J, Blackshaw S, Gaiano N, Dawson TM, Dawson VL. (2012), 'Botch promotes neurogenesis by antagonizing Notch..' Developmental Cell. 10.1016/j.devcel.2012.02.011.

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Anti-Botch Antibody (N116/14)

Anti-Botch Antibody
SKU: 75-181

Bulk Order Anti-Botch Antibody

Product Details

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