Anti-Botch Antibody (N116/14)

Name: Anti-Botch Antibody
Brand: NeuroMab
SKU: 75-181
Price: 329.0 USD
Availability: InStock

Our Anti-Botch mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N116/14. It detects human, mouse, and rat Botch, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Adult rat hippocampus immunohistochemistry.

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Myc- tagged Botch1 and untagged Kv2.1 plasmids and probed with N116/14 (left panel) and K89/34 (right panel) TC supe.

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

SKU: 75-181

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Botch

Target Description

Cation transport regulator-like protein 1, Glutathione-Specific Gamma-Glutamylcyclotransferase 1 or Botch1 is encoded by the gene CHAC1. Chac1 is a member of the gamma-glutamylcyclotransferase family. It is an enzyme that acts to catalyze the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Chac1 is involved in neuronal differentiation by deglycination of the Notch receptor, which prevents receptor maturation and inhibits Notch signaling. CHAC1 is expressed in tissues including brain (forebrain and anterior spinal cord), and heart. Diseases associated with CHAC1 include Ovarian Embryonal Carcinoma and Ovarian Cancer.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N116/14

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Chac1 Botch Npg7

Molecular Weight

25 kDa

Antigen

Fusion protein amino acids 1-222 (full-length) of rat Botch1 (accession number B3STU3.1) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2079324

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.109

Dilution Ranges

WB: 1:2000

Conjugate

Unconjugated

Specificity

No cross-reactivity against Botch2/Chac2

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Glutathione-specific gamma-glutamylcyclotransferase 1 (Gamma-GCG 1) (EC 4.3.2.7) (Blocks Notch protein) (Botch) (Cation transport regulator-like protein 1) (Neuroprotective protein 7)

UniProt ID

B3STU3

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Western Blot: Mouse | Rat

Immunocytochemistry: Mouse
PMID	Dilution	Publication
29221132	1:100	Mei, B., et al. 2017. Neuroprotection of Botch in experimental intracerebral hemorrhage in rats. Oncotarget, 95346-95360.

Western Blot: Mouse
PMID	Dilution	Publication
37800623	1:2000	Chang, YS, et al. 2023. Inhibition of the NOTCH and mTOR pathways by nelfinavir as a novel treatment for T cell acute lymphoblastic leukemia. International Journal of Oncology, .
27526673	1:500	Örd, D., et al. 2016. TRIB3 increases cell resistance to arsenite toxicity by limiting the expression of the glutathione-degrading enzyme CHAC1. Biochimica et Biophysica Acta, 2668-2680.
24767995	not listed	Chi, Z., et al. 2014. Botch is a γ-glutamyl cyclotransferase that deglycinates and antagonizes Notch.. Cell Report, 681-688.
22445366	not listed	Chi, Z., et al. 2012. Botch promotes neurogenesis by antagonizing Notch.. Developmental Cell, 707-720.

Western Blot: Rat
PMID	Dilution	Publication
29221132	not listed	Mei, B., et al. 2017. Neuroprotection of Botch in experimental intracerebral hemorrhage in rats. Oncotarget, 95346-95360.

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Anti-Botch Antibody (N116/14)

SKU: 75-181

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Botch Antibody

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