Anti-Beta4-Spectrin Antibody (N393/76)

Name: Anti-Beta4-Spectrin Antibody
Brand: NeuroMab
SKU: 75-377
Price: 361.0 USD
Availability: InStock

from NeuroMab Datasheet

11 Citations

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

N393/76

Applications

ICC, IHC, WB

Host Species

Mouse

Format

Purified

Gene Name

SPTBN4 KIAA1642 SPTBN3

Molecular Weight

>200 kDa

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Adult rat cerebral cortex immunohistochemistry.

Hippocampus immunohistochemistry with antigen retrieval via pepsin pretreatment showing axon initial segment and nodal staining.

Higher magnification of corpus callosum and CA1.

Immunoblot against extracts of COS cells transiently transfected with tagged Beta-spectrins or untagged Kv2.1 plasmid probed with N393/76 (left) or 19E10 (right) TC supe.

Adult rat cerebral cortex immunohistochemistry.

Volume: 100 µL

Variant

Price:

$361

Quantity:

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Product Details

Target

Spectrin beta chain, non-erythrocytic 4 (Beta-IV spectrin) (Spectrin, non-erythroid beta chain 3)

Target Description

Spectrin beta chain, non-erythrocytic 4, or ß4 spectrin, is a member of the spectrin family of cytoskeletal proteins. It is found in the cytoskeleton and cell cortex of cells in the brain and pancreatic islets. It is also expressed in skeletal muscule. ß4 spectrin functions as a scaffold protein that links the actin cytoskeleton to the plasma membrane. It is involved in cell shape and organization. Mutations in the ß4 spectrin gene are associated with congenital myopathy, and neurodevelopmental disorder with hypotonia, neuropathy and deafness (NEDHND).

Format

Purified

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N393/76

Isotype

IgG2b

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

SPTBN4 KIAA1642 SPTBN3

Molecular Weight

>200 kDa

Antigen

Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin (also known as BetaIV-spectrin sigma1, Nonerythrocytic spectrin beta chain 4, Non-erythroid spectrin beta chain 3, SPTBN4, SPTBN3, Spnb4, KIAA1642, EMBL BAB83243.1 and Ensembl ENSRNOP00000028393, accession number Q9H254); Mouse: 98% identity (209/212 amino acids identical); Rat: 98% identity (209/212 amino acids identical); 60% identity with Beta2-spectrin

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2315818

Storage

Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Buffer

10mM Tris, 50mM Sodium Chloride, 0.065 % Sodium Azide pH 7.4

Conjugate

Unconjugated

Specificity

Does not cross-react with other Beta-spectrins

Quality Control

Each new lot of this antibody is tested to confirm that it shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from opening

Synonyms

Spectrin beta chain, non-erythrocytic 4 (Beta-IV spectrin) (Spectrin, non-erythroid beta chain 3)

UniProt Details

UniProt (Human): Q9H254
UniProt (Immunogen Species): Q9H254

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Immunohistochemistry: Mouse | Rat
Western Blot: Mouse | Rat

Immunocytochemistry: Mouse
PMID	Dilution	Publication
36604600	1:500	Smalley, J.L., et al. 2023. Spectrin-beta 2 Facilitates the Selective Accumulation of GABAA Receptors at Somatodendritic Synapses. Communications Biology, 11.
35411984	1:500	Wang, Y., et al. 2022. Glucose Increases the Length and Spacing of the Lattice Structure of the Axon Initial Segment. Microscopy Research and Technique, 2679-2691.
34534742	not listed	Wang, Y., et al. 2021. The largest isoform of Ankyrin-G is required for lattice structure of the axon initial segment. Biochemical and biophysical research communications, 28-34.
32125730	not listed	Jarjour, A.A., et al. 2020. The formation of paranodal spirals at the ends of CNS myelin sheaths requires the planar polarity protein Vangl2. Glia, 1840-1858.
29398366	not listed	Jacko, M., et al. 2018. Rbfox Splicing Factors Promote Neuronal Maturation and Axon Initial Segment Assembly. Neuron, 853-868.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
31591156	1:200	Sozmen, E.G., et al. 2019. White matter stroke induces a unique oligo-astrocyte niche that inhibits recovery. Journal of Neuroscience, 39(47), pp.9343-9359.. Journal of Neuroscience, 9343-9359.
31532918	1:200	Di Re, J., et al. 2019. Imaging of the Axon Initial Segment. Current Protocols in Neuroscience, e78.

Immunohistochemistry: Rat
PMID	Dilution	Publication
32736374	1:250	Werginz, P., et al. 2020. The relationship between morphological properties and thresholds to extracellular electric stimulation in α RGCs. Journal of Neural Engineering, 45015.
32553116	1:250	Hanemaaijer, N.A., et al. 2020. Ca 2+ entry through Na V channels generates submillisecond axonal Ca 2+ signaling. Elife, e54566.

Western Blot: Mouse
PMID	Dilution	Publication
36604600	1:1000	Smalley, J.L., et al. 2023. Spectrin-beta 2 Facilitates the Selective Accumulation of GABAA Receptors at Somatodendritic Synapses. Communications Biology, 11.
31910261	not listed	Lee, M., et al. 2020. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. Journal of Cell Biology, e201903033.
29956586	1:500	Dash, B., et al. 2018. Nonmuscle myosin II isoforms interact with sodium channel alpha subunits. . Molecular pain, .

Western Blot: Rat
PMID	Dilution	Publication
28785724	1:1000	Kneynsberg, A., et al. 2017. Aging Does Not Affect Axon Initial Segment Structure and Somatic Localization of Tau Protein in Hippocampal Neurons of Fischer 344 Rats. eNeuro, .

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Anti-Beta4-Spectrin Antibody (N393/76)

Anti-Beta4-Spectrin Antibody
SKU: 75-377

Bulk Order Anti-Beta4-Spectrin Antibody

Product Details

Product Specific References for Applications and Species

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Anti-Beta4-Spectrin Antibody (N393/76)

Anti-Beta4-Spectrin Antibody SKU: 75-377

Bulk Order Anti-Beta4-Spectrin Antibody

Product Details

Product Specific References for Applications and Species

Anti-Beta4-Spectrin Antibody
SKU: 75-377