Anti-Beta-nerve growth factor (beta-NGF) Antibody

Name: Anti-Beta-nerve growth factor (beta-NGF) Antibody
Brand: Biosensis
SKU: S-052-500
Price: 327.0 USD
Availability: InStock

Our Anti-Beta-nerve growth factor (beta NGF) sheep polyclonal primary antibody detects avian, human, mouse, and rat Beta-nerve growth factor (beta NGF), and is IgG. It is validated for use in ELISA, IHC-Frozen, Neutralization, WB.

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, Neutralize, WB

Host Species

Sheep

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Data Sheet

SKU: S-052-500

Volume/Size: 500 µg

Variant

Price:

$327.00

Quantity:

Product Details

Target

Beta-nerve growth factor (beta NGF)

Scientific Background

FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.

Format

IgG

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, IHC, Neutralize, WB

Host Species

Sheep

Immunogen

Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Storage

Spin vial briefly before opening. Reconstitute in 500 µL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.

Physical State

Lyophilized

Production Notes

Protein G purified IgG

Buffer

Lyophilized from a solution containing PBS pH 7.4

Dilution Ranges

WB: 1-3 µg/mL
IHC: 1-3 µg/mL
ELISA: 1-3 µg/mL

Application Details

A concentration of 1-3 µg/mL is recommended for inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro.

Conjugate

Unconjugated

Specificity

A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Beta-nerve growth factor

UniProt ID

P01139

Shipping

25°C (ambient)

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Anti-Beta-nerve growth factor (beta-NGF) Antibody

SKU: S-052-500

Product Details

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