Our beta Catenin rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects beta Catenin and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.
Immunofluorescence of transfected COS-7 cells specifically labeling positive beta Catenin cells using our anti-beta Catenin antibody (1:200, red) and anti-FLAG antibody (green). The yellow color results from the overlap of cells labeled with both antibodies. DNA is labeled with DAPI (blue).
beta Catenin, considered a moonlighting protein, is a component of mammalian cell adhesion complex in the cytoplasm along with playing a key downstream component of the canonical Wingless Int-1 (Wnt) signaling pathway in the nucleus (McTrea P.D et al, 1991 and Kemler R. et al, 1993). As part of a protein complex that forms adheren junctions, beta Catenin is necessary for the creation and maintenance of epithelial cell layers and barriers (Brembeck F.H. et al, 2006). beta Catenin is responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete (Brembeck F.H. et al, 2006). The Wnt/beta Catenin signaling pathway plays important roles in mammalian development (Logan C.Y. et al, 2004 ) and irregular regulation of this signaling pathway is associated with human diseases and cancers (Moon R.T., 2004). HIPK2 is a negative regulator of the Wnt signaling via the direct phosphorylation of beta Catenin at ser33/37 causing degradation (Kim E.A. et al, 2010). Additionally, stabilization of beta Catenin has been shown when AMPK phosphorylates at ser552 regulating energy metabolism to cell differentiation and development by enhancing the beta Catenin/TCF mediated transcription within the Wnt/betaCatenin signaling pathway (Zhao J. et al, 2010).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ICC, WB
Rabbit
CTNNB1
92 kDa
Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat beta Catenin, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
Human
AB_2927666
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification on a column made with the C-terminal peptide used as antigen.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
ICC: 1:200
Unconjugated
Specific for endogenous levels of the ~92 kDa beta Catenin protein. Immunolabeling of the beta Catenin band is completely blocked by preadsorption of the antibody with the peptide used as antigen.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
