BCAT1-3 1782 × 452px Staining of COS7 cells overexpressing FLAG-tagged beta-catenin with: chicken anti-beta catenin antibody BCAT (left panel in green) showing both endogenous staining of beta catenin in COS7 cells and staining of overexpressed nuclear beta-catenin (white arrow), anti-FLAG staining of overexpressed nuclear beta-catenin (middle panel in red), and overlay (right panel) with DAPI staining of nuclei to show all cells.
Bulk Order Anti-Beta-Catenin Antibody
Beta-catenin (CTNNB1) is an adherens junction protein critical for the establishment and maintenance of epithelial layers while at the same time is also a transcription factor and key downstream component of the canonical Wnt signaling pathway which regulates cell fate during development and also contributes to malignant transformation in various cancers.
Affinity Purified IgY
ICC, IHC, WB
KLH-conjugated peptide encoding sequence near C-terminus of protein
Human, Mouse, Rat
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.
Phosphate-buffered saline (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot on lysate from cells expressing endogenous beta catenin.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
12 months from receipt of product
UniProt (Human): P35222
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