Anti-Beta-Catenin Antibody

Name: Anti-Beta-Catenin Antibody
Brand: Aves Labs
SKU: BCAT-0100
Price: 440.0 USD
Availability: InStock

from Aves Labs Datasheet

Trial Size

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

Format

Affinity Purified IgY

Gene Name

CTNNB1

Molecular Weight

90 kDa

Go to Full Product Details

BCAT1-3 1782 × 452px Staining of COS7 cells overexpressing FLAG-tagged beta-catenin with: chicken anti-beta catenin antibody BCAT (left panel in green) showing both endogenous staining of beta catenin in COS7 cells and staining of overexpressed nuclear beta-catenin (white arrow), anti-FLAG staining of overexpressed nuclear beta-catenin (middle panel in red), and overlay (right panel) with DAPI staining of nuclei to show all cells.

Staining of paraffin wax mouse lung sections using beta-catenin antibody (Cat. No. BCAT, 1:100 dilution) with antigen retrieval and biotinylated secondary antibodies plus streptavidin. Image courtesy of Kuan Zhang (UCSF).

Western blot staining of endogenous beta-catenin in HEK-293T cells using chicken anti-beta catenin antibody BCAT.

BCAT1-3 1782 × 452px Staining of COS7 cells overexpressing FLAG-tagged beta-catenin with: chicken anti-beta catenin antibody BCAT (left panel in green) showing both endogenous staining of beta catenin in COS7 cells and staining of overexpressed nuclear beta-catenin (white arrow), anti-FLAG staining of overexpressed nuclear beta-catenin (middle panel in red), and overlay (right panel) with DAPI staining of nuclei to show all cells.

Volume: 500 µL

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Price:

$440

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Product Details

Target

Beta Catenin

Target Description

Beta-catenin (CTNNB1) is an adherens junction protein critical for the establishment and maintenance of epithelial layers while at the same time is also a transcription factor and key downstream component of the canonical Wnt signaling pathway which regulates cell fate during development and also contributes to malignant transformation in various cancers.

Format

Affinity Purified IgY

Concentration

0.2 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

CTNNB1

Molecular Weight

90 kDa

Antigen

KLH-conjugated peptide encoding sequence near C-terminus of protein

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2924439

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.

Buffer

Phosphate-buffered saline (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:500-1:1000
IHC: 1:100-1:500
ICC: 1:100-1:500

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot on lysate from cells expressing endogenous beta catenin.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

UniProt Details

UniProt (Human): P35222

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Anti-Beta-Catenin Antibody