Array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex with L124/59 (magenta), rabbit mAb PSD-95 (Cell Signaling #3450, green) and Hoechst nuclear stain (blue). The insert shows three consecutive sections through the synapse that is marked with a white box..
Bassoon is a large (420-kDa) scaffolding protein specifically localized at the active zone of presynaptic nerve terminals. Bassoon is involved in the development of presynaptic terminals and in the regulation of neurotransmitter release at both excitatory and inhibitory brain synapses (Richter K. et al., 1999).
AT, ICC, IHC, WB
400 kDa for full-length protein, many smaller bands possible depending on sample used
Fusion protein amino acids 157-517 (zinc finger domains) of rat Bassoon (also known as Zinc finger protein 231, BSN, ZNF231 and KIAA0434, accession number O88778); Mouse: 96% identity (349/363 amino acids identical); Human: 90% identity (325/361 amino acids identical); 40% identity with Piccolo
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.