Anti-Autophagy-related protein 4 homolog C (ATG4C) Antibody

Name: Anti-Autophagy-related protein 4 homolog C (ATG4C) Antibody
Brand: Biosensis
SKU: R-156-100
Price: 327.0 USD
Availability: InStock

Our Anti-Autophagy-related protein 4 homolog C (ATG4C) rabbit polyclonal primary antibody detects human and rat Autophagy-related protein 4 homolog C (ATG4C), and is whole serum. It is validated for use in IHC-Frozen, WB.

Confocal microscopy on immunofluorescently detected APG4C in HL60 cell line using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for APG4C appear in red. The cells were then counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.

Western blot (non-reducing) on Jurkat cell lysate using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100).

Western blot on Peripheral Blood Mononuclear Cells (PMBC) lysate using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1: 100 (ECL).

Western blot under reducing conditions on Olfactory cell line (Odora) lysate using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1:100.

Western blot under reducing conditions on rat liver lysate using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1:100.

Western blot under reducing conditions on 293 cell lysate using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1:100.

Confocal microscopy on immunofluorescently detected APG4C in HL60 cell line using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for APG4C appear in red. The cells were then counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.

Click on image to zoom

Species Reactivity

Human, Rat

Applications

IHC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-156-100

Volume/Size: 100 µL

Variant

Price:

$327.00

Quantity:

Product Details

Target

Autophagy-related protein 4 homolog C (ATG4C)

Scientific Background

FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm (Probable). SIMILARITY: Belongs to the peptidase C54 family.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

IHC, WB

Host Species

Rabbit

Immunogen

A synthetic peptide (CTVYNSDVIDKQ) corresponding to the C-terminal of human ATG4C protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from ATG4C protein in mouse and rat.

Immunogen Species

Human

Species Reactivity

Human, Rat

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Dilution Ranges

WB: 1:100-1:2000
IHC: 1:100-1:2000

Conjugate

Unconjugated

Specificity

IHC, ELISA and WB confirmed the specificity for ATG4C. Human, rat. Other species not yet tested.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Cysteine protease ATG4C; Autophagy-related protein 4 homolog C; Autophagin-3; Autophagy-related cysteine endopeptidase 3; AUT-like 3 cysteine endopeptidase; ATG4C; APG4C; AUTL3;

UniProt ID

Q96DT6

Shipping

25°C (ambient)

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Anti-Autophagy-related protein 4 homolog C (ATG4C) Antibody

SKU: R-156-100

Product Details

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