Anti-Atrogin-1 Antibody (M314)

Name: Anti-Atrogin-1 Antibody
Brand: PhosphoSolutions
SKU: AM3141
Price: 329.0 USD
Availability: InStock

7 Citations

Our Atrogin-1 rat rat monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Atrogin-1 and is antigen affinity purified. It is great for use in WB.

Western blot image of human recombinant Atrogin 1 (lanes 1-6). The blot was probed with rabbit polyclonal Atrogin-1 (lanes 1-3) and rat monoclonal Atrogin-1 (lanes 4-6) at 1:1000 (lanes 1 & 4), 1:2000 (lanes 2 & 5), and 1:4000 (lanes 3 & 6).

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rat

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Data Sheet

SKU: AM3141

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Product Details

Target

Atrogin-1

Target Description

Atrogin-1/Muscle Atrophy F-box (MAFbx) is an E3 ubiquitin ligase that mediates proteolysis events that occur during muscle atrophy. This ATP-dependent ubiquitin-mediated proteolysis occurs in response to a variety of catabolic states in muscle. Atrogin is expressed in heart and skeletal muscle, and is upregulated during muscle atrophy. In addition, Atrogin expression increases in C2C12 myotubes after stimulation with cytokines. Atrogin is thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. Atrogin interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD. Mice deficient in Atrogin are resistant to muscle atrophy.

Format

Antigen Affinity Purified

Clonality

Monoclonal

Clone

M314

Applications

Host Species

Rat

Gene Name

Fbxo32

Molecular Weight

Antigen

Clone M314 was generated from a synthetic peptide (coupled to carrier protein) corresponding to amino acids from the N-terminal region of mouse Atrogin. This sequence is highly homologous to human and rat Atrogin (F-box only protein 32).

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500

Conjugate

Unconjugated

Specificity

This antibody detects a full length human recombinant Atrogin 1 on SDS-PAGE immunoblots.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

MAFbx, FBX32, Atrogin

UniProt Details

UniProt (Human): Q969P5
UniProt (Immunogen Species): Q9CPU7

Product Specific References for Applications and Species

Immunocytochemistry: Human
Western Blot: Human | Mouse | Rat

Immunocytochemistry: Human
PMID	Dilution	Publication
38267369	1:100	Gerosa, L, et al. 2024. Murine myoblasts exposed to SYUIQ-5 acquire senescence phenotype and differentiate into sarcopenic-like myotubes, an in vitro study. The journals of gerontology. Series A, Biological sciences and medical sciences, .

Western Blot: Human
PMID	Dilution	Publication
38267369	1:1000	Gerosa, L., et al. 2024. Murine myoblasts exposed to SYUIQ-5 acquire senescence phenotype and differentiate into sarcopenic-like myotubes, an in vitro study. The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences, .
33909501	not listed	Allen, SL, et al. 2021. The effect of young and old ex vivo human serum on cellular protein synthesis and growth in an in vitro model of aging. American journal of physiology. Cell physiology, C26-C37.

Western Blot: Mouse
PMID	Dilution	Publication
36454675	1:1000	Kincheloe, GN, et al. 2023. Loss of 4E-BPs prevents the hindlimb immobilization-induced decrease in protein synthesis in skeletal muscle. Journal of applied physiology (Bethesda, Md. : 1985), 72-83.
35704697	1:1000	Nishimura, Y, et al. 2022. Ubiquitin E3 ligase Atrogin-1 protein is regulated via the rapamycin-sensitive mTOR-S6K1 signaling pathway in C2C12 muscle cells. American journal of physiology. Cell physiology, C215-C225.
35406665	not listed	Allen, SL, et al. 2022. The Effect of Ex Vivo Human Serum from Liver Disease Patients on Cellular Protein Synthesis and Growth. Cells, 1098.

Western Blot: Rat
PMID	Dilution	Publication
28336537	1:1000	Baehr, L, et al. 2017. Muscle-specific and age-related changes in protein synthesis and protein degradation in response to hindlimb unloading in rats. Journal of applied physiology (Bethesda, Md. : 1985), 1336-1350.

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Anti-Atrogin-1 Antibody (M314)

SKU: AM3141

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Atrogin-1 Antibody

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