Anti-ATF2 (Thr52) Antibody

Name: Anti-ATF2 (Thr52) Antibody
Brand: PhosphoSolutions
SKU: p115-52
Price: 430.0 USD
Availability: InStock

4 Citations

Our Anti-ATF2 (Thr52) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse ATF2 (Thr52) and is antigen affinity purified from pooled serum. It is great for use in WB.

Western blot of UACC903 melanoma cell line lysate (DMSO) showing specific immunolabeling of the ~ 72 kDa ATF2 protein phosphorylated at Thr52. Immunolabeling is reduced by treatment of the lysate with the PKC catalytic inhibitor Gö6850. Image courtesy of Eric Lau and Ze’ev Ronai.

Species Reactivity

Human, Mouse

Applications

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: p115-52

Volume: 100 µL

Variant

Price:

$430.00

Quantity:

Product Details

Target

ATF2 (Thr52)

Target Description

The transcription factor ATF2 is a member of the ATF/CREB family of leucine zipper proteins. In response to stress stimuli, it activates a variety of gene targets that are involved in oncogenesis, and has been correlated with maintenance of a cancer cell phenotype (Vlahopoulos et al., 2008). Inhibiting ATF2 impedes melanoma development and elicits tumor suppressor function (Bhoumik et al., 2008). To act as a tumor suppressor, ATF2 must localize at the mitochondria, and phosphorylation at Thr-52 by PKCε regulates this translocation (Lau et al., 2012).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

ATF2

Molecular Weight

74 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr52 of human ATF2, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Mouse

Antibody Registry ID

AB_2492045

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:250

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~72 kDa ATF2 protein phosphorylated at Thr52. Immunolabeling of the ATF2 band is reduced by treatment of the cells with the PKC catalytic inhibitor Gö6850.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr52

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Activating transcription factor 2 antibody, Activating transcription factor 2 splice variant ATF2 var2 antibody, ATF 2 antibody, Atf-2 antibody, Atf2 antibody, ATF2 protein antibody, ATF2_HUMAN antibody, cAMP Response Element Binding Protein 2 antibody, cAMP response element binding protein CRE BP1 antibody, cAMP response element-binding protein CRE-BP1 antibody, cAMP responsive element binding protein 2, formerly antibody, cAMP-dependent transcription factor ATF-2 antibody, cAMP-responsive element-binding protein 2 antibody, CRE BP1 antibody, CRE-BP antibody, CREB 2 antibody, CREB-2 antibody, CREB2 antibody, CREBP1 antibody, Cyclic AMP dependent transcription factor ATF 2 antibody, Cyclic AMP-dependent transcription factor ATF-2 antibody, Cyclic AMP-responsive element-binding protein 2 antibody, D130078H02Rik antibody, D18875 antibody, HB 16 antibody, HB16 antibody, Histone acetyltransferase ATF2 antibody, MGC105211 antibody, MGC105222 antibody, MGC111558 antibody, MGC142504 antibody, mXBP antibody, MXBP protein antibody, Tg(Gzma-Klra1)7Wum antibody, TREB 7 antibody, TREB7 antibody

UniProt Details

UniProt (Human): P15336
UniProt (Immunogen Species): P15336

NCBI Gene ID

1386

Product Specific References for Applications and Species

Western Blot: Human | Mouse

Western Blot: Human
PMID	Dilution	Publication
25728676	not listed	Lau, E., et al. 2015. Transcriptional repression of IFNβ1 by ATF2 confers melanoma resistance to therapy. Oncogene, 34(46), p.5739-48.
23589174	not listed	Varsano, T., et al. 2013. Inhibition of melanoma growth by small molecules that promote the mitochondrial localization of ATF2. Clinical Cancer Research, 19(10), 2710-2722.
22304920	not listed	Lau, E., et al. 2013. PKCε promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria. Cell, 148(3), pp.543-555.

Western Blot: Mouse
PMID	Dilution	Publication
30497386	1:200	Kumar, V., et al. 2018. PKCε phosphorylation regulates the mitochondrial translocation of ATF2 in ischemia-induced neurodegeneration. BMC neuroscience, 19(1), p.76.

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Product Specific Protocols

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Anti-ATF2 (Thr52) Antibody

SKU: p115-52

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-ATF2 (Thr52) Antibody

Product Specific Protocols

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