Our Anti-ATF2 (Ser490,498) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse ATF2 (Ser490,498) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.
Western blot of human melanoma cells incubated with varying doses of the radiomimetic drug NCS showing specific immunolabeling of the ~74 kDa ATF2 protein phosphorylated at Ser490 and Ser498.
The activating transcription factor ATF2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (Maekawa et al., 1989). ATF2 has been implicated in the transcriptional regulation of a number of genes including cytokines, cell cycle control and apoptosis. Various forms of cellular stress, including inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF2 (Ivanov et al., 2003; Morton et al., 2004). Stress induced ATFdependent transcription is dependent on phosphorylation of ATF (Fuchs et al., 2000); Morton et al., 2004). Serine 490 and serine 498 are novel phosphorylation sites on ATF that have recently been identified. ATF2 is particularly abundant in the brain and the ATF2 family of transcription factors is considered an important substrate of signals upstream of the activation of genes associated with neuronal growth and differentiation (Karin and Hunter, 1995). ATF expression has also been linked to the depression in humans (Laifenfeld et al., 2004).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ICC, IHC, WB
Rabbit
ATF2
74 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser490/498 of human ATF2, conjugated to keyhole limpet hemocyanin (KLH).
Human, Mouse
Rat
AB_2492044
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
IHC: 1:1000
ICC: 1:500
Unconjugated
Specific for endogenous levels of the ~74 kDa ATF2 protein phosphorylated at Ser490,498. The antibody also recognizes the phosphorylated ~54 kDa splice form of ATF2.
Phosphorylated
Ser490,498
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Muñoz, D.P., et al. 2013. An autonomous chromatin/DNA-PK mechanism for localized DNA damage signaling in mammalian cells. Nucleic acids research 41.5 (2013): 2894-2906.
