Anti-ATF2 (Ser490,498) Antibody

Name: Anti-ATF2 (Ser490,498) Antibody
Brand: PhosphoSolutions
SKU: p115-4908
Price: 456.0 USD
Availability: InStock

Our Anti-ATF2 (Ser490,498) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse ATF2 (Ser490,498) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.

Western blot of human melanoma cells incubated with varying doses of the radiomimetic drug NCS showing specific immunolabeling of the ~74 kDa ATF2 protein phosphorylated at Ser490 and Ser498.

Click on image to zoom

Species Reactivity

Human, Mouse

Applications

ELISA, ICC, IHC, WB

Host Species

Rabbit

Go to Full Product Details

Data Sheet

Trial Size

Pooled Serum

SKU: p115-4908

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

ATF2 (Ser490,498)

Scientific Background

The activating transcription factor ATF2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (Maekawa et al., 1989). ATF2 has been implicated in the transcriptional regulation of a number of genes including cytokines, cell cycle control and apoptosis. Various forms of cellular stress, including inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF2 (Ivanov et al., 2003; Morton et al., 2004). Stress induced ATFdependent transcription is dependent on phosphorylation of ATF (Fuchs et al., 2000); Morton et al., 2004). Serine 490 and serine 498 are novel phosphorylation sites on ATF that have recently been identified. ATF2 is particularly abundant in the brain and the ATF2 family of transcription factors is considered an important substrate of signals upstream of the activation of genes associated with neuronal growth and differentiation (Karin and Hunter, 1995). ATF expression has also been linked to the depression in humans (Laifenfeld et al., 2004).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, IHC, WB

Host Species

Rabbit

Gene Name

ATF2

Molecular Weight

74 kDa

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser490/498 of human ATF2, conjugated to keyhole limpet hemocyanin (KLH).

Immunogen Species

Human

Species Reactivity

Human, Mouse

Expected Reactivity

Rat

Antibody Registry ID

AB_2492044

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
IHC: 1:1000
ICC: 1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~74 kDa ATF2 protein phosphorylated at Ser490,498. The antibody also recognizes the phosphorylated ~54 kDa splice form of ATF2.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser490,498

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Activating transcription factor 2; Activating transcription factor 2 splice variant ATF2 var2; ATF 2; Atf-2; Atf2; ATF2 protein; ATF2_HUMAN; cAMP Response Element Binding Protein 2; cAMP response element binding protein CRE BP1; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2; formerly; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; CRE BP1; CRE-BP; CREB 2; CREB-2; CREB2; CREBP1; Cyclic AMP dependent transcription factor ATF 2; Cyclic AMP-dependent transcription factor ATF-2; Cyclic AMP-responsive element-binding protein 2; D130078H02Rik; D18875; HB 16; HB16; Histone acetyltransferase ATF2; MGC105211; MGC105222; MGC111558; MGC142504; mXBP; MXBP protein; Tg(Gzma-Klra17Wum; TREB 7; TREB7; Tg(Gzma-Klra1)7Wum

UniProt ID

P15336
UniProt Summary: Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro (PubMed:10821277). In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type. {ECO:0000269|PubMed:10821277, ECO:0000269|PubMed:15916964, ECO:0000269|PubMed:18397884, ECO:0000269|PubMed:22304920}.

NCBI Gene ID

1386

Shipping

Blue Ice

Western Blot Protocol: Download

Recover password

Create my account

Anti-ATF2 (Ser490,498) Antibody

SKU: p115-4908

Product Details

Bulk Order Anti-ATF2 (Ser490,498) Antibody

Related Products

Recently Viewed