Anti-Ataxin-1, 11NQ Antibody (N76/8)

Name: Anti-Ataxin-1, 11NQ Antibody
Brand: NeuroMab
SKU: 75-117
Price: 329.0 USD
Availability: InStock

5 Citations

Our Anti-Ataxin-1, 11NQ mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N76/8. It is KO validated, detects human, mouse, and rat Ataxin-1, 11NQ, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Transfected cell immunoblot: COS cells transiently transfected with Ataxin-1 and Kv2.1 plasmids and probed with N76/8 TC supe.

Adult rat brain immunoblot: extracts of cerebella from Ataxin-1 KO and WT mice and probed with N76/8 (left) or K89/41 (right) TC supe.

Adult rat cerebellum immunohistochemistry.

Adult rat hippocampus immunohistochemistry.

Transfected cell immunoblot: COS cells transiently transfected with Ataxin-1 and Kv2.1 plasmids and probed with N76/8 TC supe.

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-117

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Ataxin-1, 11NQ

Scientific Background

Ataxin1, also known as spinocerebellar ataxia type 1 protein homolog, is a highly conserved DNA-binding protein. Ataxin1 is expressed in brain and can be found at high levels in the cortex and the hypothalamus in neurons. It is also expressed in other tissues. Within the cell, it is highly expressed in the nucleus, and can also be found in the cytoplasm. Mutations in the ataxin-1 gene cause spinocerebellar ataxia type 1.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N76/8

Isotype

IgG2b

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Atxn1 Sca1

Molecular Weight

85 kDa

Immunogen

Synthetic peptide amino acids 164-197 (ATTPSQRSQLEAYSTLLANMGSLSQAPGHKVEPP) of mouse ataxin-1 (accession number P54254)

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10673960

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.45

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Ataxin-1 (Spinocerebellar ataxia type 1 protein homolog)

UniProt ID

P54254

Shipping

Shipped on ice packs

Product Specific References for Applications and Species

Co-IP: Human
Immunohistochemistry: Mouse
Western Blot: Mouse

Co-Immunoprecipitation: Human
PMID	Dilution	Publication
20037628	not listed	de Chiara, C., et al. 2009. Phosphorylation of S776 and 14-3-3 binding modulate ataxin-1 interaction with splicing factors.. PLoS One, e8372.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
31442405	not listed	Suh, J., et al. 2019. Loss of Ataxin-1 Potentiates Alzheimer's Pathogenesis by Elevating Cerebral BACE1 Transcription. Cell, 1159-1175.

Western Blot: Mouse
PMID	Dilution	Publication
31442405	not listed	Suh, J., et al. 2019. Loss of Ataxin-1 Potentiates Alzheimer's Pathogenesis by Elevating Cerebral BACE1 Transcription. Cell, 1159-1175.
27306906	not listed	Cvetanovic, M., et al. 2017. Mutant ataxin-1 inhibits neural progenitor cell proliferation in SCA1.. The Cerebellum, 340-347.
21245341	not listed	Jafar-Nejad, P., et al. 2011. Regional rescue of spinocerebellar ataxia type 1 phenotypes by 14-3-3epsilon haploinsufficiency in mice underscores complex pathogenicity in neurodegeneration.. PNAS USA, 2142-2147.
20097758	1:1000	Zhang, C., et al. 2010. Loss of function of ATXN1 increases amyloid beta-protein levels by potentiating beta-secretase processing of beta-amyloid precursor protein.. The Journal of Biological Chemistry, 8515-8526.

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Anti-Ataxin-1, 11NQ Antibody (N76/8)

SKU: 75-117

Product Details

Product Specific References for Applications and Species

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