Anti-Arl13b Antibody (N295B/66)

from NeuroMab Datasheet KO Validated KO Validated Trial Size Trial Size
183 Citations

Available Conjugates

490
550
594
650

Detect Arl13b with this antibody validated for IHC, WB, ICC.

Primary Antibody
Human, Mouse, Non-Human Primate, Rat, Zebrafish
N295B/66
ICC, IHC, WB
Mouse
Purified by Protein A chromatography
Arl13b Arl2l1
50 kDa

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Immunofluorescence of the ventricular zone of E14 mouse brain (frozen section) labeled with Anti-Arl13b (red, Cat No. 75-287, 1:1000) and CEP152 (Green). The blue is DAPI staining of nuclear DNA. Image kindly provided by Koh-ichi Nagata, Nagoya University Graduate School of Medicine.
Immunofluorescence staining of adult rat brain sections with N295B/66 (red, cilia) and N147/6 (green) TC supe.
Adult rat brain immunohistochemistry of ciliated cells surrounding the lateral ventricle (LV).
Immunoblot versus crude membranes from adult rat brain (RBM) and pellet/lysate fractions of wild-type (WT) and Arl13b knockout (KO) mouse embryonic fibroblasts (MEF) probed with N295B/66 TC supe (left), rabbit polyclonal (middle) and N52A/42 TC supe (right). MEF cells courtesy of Laura Mariani and Tamara Caspary (Emory University SOM).
Immunostaining of KIF24-depleted Panc1 cells in serum-starved medium treated with DMSO or 1 μM SAG for 48 h. Cells were immunostained with anti-ARL13B (green) and anti-GPR161 (red) antibodies, DNA was stained with Hoechst (blue). Image from publication CC-BY-4.0. PMID: 35803737
Semi-thin resin sections of the mouse retina were immunolabeled with affinity-purified mouse monoclonal antibody against Arl13b (NeuroMab) (H1,I1), rabbit polyclonal antibody against RIBEYE (U2656) (H2) and rabbit polyclonal antibody against PSD-95 (I2). Signals are overlaid in (H3,I3). Image from publication CC-BY-4.0. PMID: 35806143
Spinning disc confocal image identifying localization of the atypical GTPase protein ARL13B (Cat 75-287, red) in primary cilium, marked by acetylated tubulin (green), in Aquaporin 2 (magenta) positive renal collecting ducts in a 24-day old mouse. Image kindly provided by Kevin White, UTSW-Dallas.
Ciliogenesis induced mIMCD3 cells stably expressing eGFP-UBL3 WT (upper panel – arrows inside and at base of cilium) and eGFP-UBL3 C>A (middle panel – arrowheads at basal bodies/centromeres), and transiently expressing eGFP-UBL3 S>K (bottom panel – arrows inside cilium). Cilia stained with antibodies against ARL13B (cat. 75-287, 1:500; magenta). Basal bodies stained with antibodies against pericentrin (PCTN, red). Nuclei stained with DAPI (blue). Image from publication CC-BY-4.0. PMID:36672247
Representative images of immunostaining of ARL13B (cat. 75-287, 1:500; red) and K5 (green) at the base of the olfactory epithelium (OE) in WT and 123-Arl13b mice at P5. Image from publication CC-BY-4.0. PMID:36661357
Representative confocal images of WT and Dp10 mouse embryonic fibroblasts. Cells were stained with Hoechst 33342 (blue), the ciliary marker ARL13B (cat. 75-287, 1:500; green), and Actub (magenta). Image from publication CC-BY-4.0. PMID:36656118
Primary cilium formation in hTERT-RPE1 cells treated with siRNA: control (a), UBQLN4 (b), BAG6 (c), and RNF126 (d). At 72 h after transfection, hTERT-RPE1 cells were serum-starved for 24 h to trigger ciliogenesis, and the distribution of ARL13B, a marker for primary cilia, was observed (cat. 75-287 green). Control cells grew primary cilia (a), whereas those treated with knockdown of UBQLN4 (b), BAG6 (c), and RNF126 (d) failed to grow cilia. Image from publication CC-BY-4.0. PMID:37182096
Confocal images from flat mount mouse retina samples at different postnatal ages showing the peripheral or central areas of RPE. ZO-1 staining cell junctions (red), Arl13b is a ciliary marker (cat. 75-287, 1:2000; green), nuclei (blue). Magnifications of each corresponding image are shown. Image from publication CC-BY-4.0. PMID:37211572
Confocal images stained for Arl13b (cat. 75-287, 1:2000; green) and ZO-1 (red) of healthy human RPE and AMD patient. Image from publication CC-BY-4.0. PMID:37211572
Immunofluorescence of the ventricular zone of E14 mouse brain (frozen section) labeled with Anti-Arl13b (red, Cat No. 75-287, 1:1000) and CEP152 (Green). The blue is DAPI staining of nuclear DNA. Image kindly provided by Koh-ichi Nagata, Nagoya University Graduate School of Medicine.
Immunofluorescence of the ventricular zone of E14 mouse brain (frozen section) labeled with Anti-Arl13b (red, Cat No. 75-287, 1:1000) and CEP152 (Green). The blue is DAPI staining of nuclear DNA. Image kindly provided by Koh-ichi Nagata, Nagoya University Graduate School of Medicine.
Immunofluorescence staining of adult rat brain sections with N295B/66 (red, cilia) and N147/6 (green) TC supe.
Adult rat brain immunohistochemistry of ciliated cells surrounding the lateral ventricle (LV).
Immunoblot versus crude membranes from adult rat brain (RBM) and pellet/lysate fractions of wild-type (WT) and Arl13b knockout (KO) mouse embryonic fibroblasts (MEF) probed with N295B/66 TC supe (left), rabbit polyclonal (middle) and N52A/42 TC supe (right). MEF cells courtesy of Laura Mariani and Tamara Caspary (Emory University SOM).
Immunostaining of KIF24-depleted Panc1 cells in serum-starved medium treated with DMSO or 1 μM SAG for 48 h. Cells were immunostained with anti-ARL13B (green) and anti-GPR161 (red) antibodies, DNA was stained with Hoechst (blue). Image from publication CC-BY-4.0. PMID: 35803737
Semi-thin resin sections of the mouse retina were immunolabeled with affinity-purified mouse monoclonal antibody against Arl13b (NeuroMab) (H1,I1), rabbit polyclonal antibody against RIBEYE (U2656) (H2) and rabbit polyclonal antibody against PSD-95 (I2). Signals are overlaid in (H3,I3). Image from publication CC-BY-4.0. PMID: 35806143
Spinning disc confocal image identifying localization of the atypical GTPase protein ARL13B (Cat 75-287, red) in primary cilium, marked by acetylated tubulin (green), in Aquaporin 2 (magenta) positive renal collecting ducts in a 24-day old mouse. Image kindly provided by Kevin White, UTSW-Dallas.
Ciliogenesis induced mIMCD3 cells stably expressing eGFP-UBL3 WT (upper panel – arrows inside and at base of cilium) and eGFP-UBL3 C>A (middle panel – arrowheads at basal bodies/centromeres), and transiently expressing eGFP-UBL3 S>K (bottom panel – arrows inside cilium). Cilia stained with antibodies against ARL13B (cat. 75-287, 1:500; magenta). Basal bodies stained with antibodies against pericentrin (PCTN, red). Nuclei stained with DAPI (blue). Image from publication CC-BY-4.0. PMID:36672247
Representative images of immunostaining of ARL13B (cat. 75-287, 1:500; red) and K5 (green) at the base of the olfactory epithelium (OE) in WT and 123-Arl13b mice at P5. Image from publication CC-BY-4.0. PMID:36661357
Representative confocal images of WT and Dp10 mouse embryonic fibroblasts. Cells were stained with Hoechst 33342 (blue), the ciliary marker ARL13B (cat. 75-287, 1:500; green), and Actub (magenta). Image from publication CC-BY-4.0. PMID:36656118
Primary cilium formation in hTERT-RPE1 cells treated with siRNA: control (a), UBQLN4 (b), BAG6 (c), and RNF126 (d). At 72 h after transfection, hTERT-RPE1 cells were serum-starved for 24 h to trigger ciliogenesis, and the distribution of ARL13B, a marker for primary cilia, was observed (cat. 75-287 green). Control cells grew primary cilia (a), whereas those treated with knockdown of UBQLN4 (b), BAG6 (c), and RNF126 (d) failed to grow cilia. Image from publication CC-BY-4.0. PMID:37182096
Confocal images from flat mount mouse retina samples at different postnatal ages showing the peripheral or central areas of RPE. ZO-1 staining cell junctions (red), Arl13b is a ciliary marker (cat. 75-287, 1:2000; green), nuclei (blue). Magnifications of each corresponding image are shown. Image from publication CC-BY-4.0. PMID:37211572
Confocal images stained for Arl13b (cat. 75-287, 1:2000; green) and ZO-1 (red) of healthy human RPE and AMD patient. Image from publication CC-BY-4.0. PMID:37211572

Anti-Arl13b Antibody
SKU: 75-287

Volume: 100 µL
1-2 business days
Price:
$361

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Product Specific References for Applications and Species

Immunocytochemistry: Human
PMID Dilution Publication
37182096not listedTakahashi, T., et al. 2023. Protein Quality Control Machinery Supports Primary Ciliogenesis by eliminating GDP-bound Rab8-family GTPases. iScience, 106652.
371645421:200Hua, K., et al. 2023. Fixation Methods and Immunolabeling for Cilia Proteins in Ciliary and Extraciliary Locations. Methods in Cell Biology, 43-57.
366843871:2000Ning, K., et al. 2023. Characterization of Primary Cilia Formation in Human ESC-Derived Retinal Organoids. Stem Cells International, 6494486.
366722471:500Faber, S., et al. 2023. PDE6D Mediates Trafficking of Prenylated Proteins NIM1K and UBL3 to Primary Cilia. Cells, 312.
36652335not listedPalicharla, V.R., et al. 2023. Interactions Between TULP3 Tubby Domain and ARL13B Amphipathic Helix Promote Lipidated Protein Transport to Cilia. Molecular Biology of the Cell, mbcE22100473.
366064241:100Odabasi, E., et al. 2023. CCDC66 Regulates Primary Cilium Length and Signaling via Interactions with Transition Zone and Axonemal Proteins. Journal of Cell Science, jcs260327.
358037371:1000Mashima, Y., et al. 2022. KIF24 Depletion Induces Clustering of Supernumerary Centrosomes in PDAC Cells. Life Science Alliance, .
352894771:100Jabali, A., et al. 2022. Human cerebral organoids reveal progenitor pathology in EML1-linked cortical malformation. EMBO reports, e54027.
352538371:250Afanasyeva, T.A.V., et al. 2022. PCARE requires coiled coil, RP62 kinase binding, and EVH1 domain binding motifs for ciliary expansion. Human molecular genetics, .
346781631:300Rivera-Molina, F.E., et al. 2021. Exocyst complex mediates recycling of internal cilia. Current biology : CB, 5580-5589.e5.
337272501:2000Dhekne, H.S., et al. 2021. LRRK2-phosphorylated Rab10 sequesters Myosin Va with RILPL2 during ciliogenesis blockade. Life Science Alliance, e202101050.
33206585not listedFailler, M., et al. 2021. Whole-genome screen identifies diverse pathways that negatively regulate ciliogenesis. Mol Biol Cell., 169-185.
332512151:1000Kobayashi, T., et al. 2020. CEP164 Deficiency Causes Hyperproliferation of Pancreatic Cancer Cells. Frontiers in Cell and Developmental Biology, 587691.
325555911:500Aydin, Ö.Z., et al. 2020. Acute inhibition of centriolar satellite function and positioning reveals their functions at the primary cilium. PLoS Biology, e3000679.
313884141:300Lauring, M.C., et al. 2019. New software for automated cilia detection in cells (ACDC). Cilia, 1.
310237191:1000Odabasi, E., et al. 2019. Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation. EMBO Reports, e47723.
306838961:300Insinna, C., et al. 2019. Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport. Nature Communications, 428.
306221761:500Zhang, Q., et al. 2019. Disruption of RPGR protein interaction network is the common feature of RPGR missense variations that cause XLRP. PNAS: USA, 1353-1360.
303981481:1000Dhekne, H.S., et al. 2018. A pathway for Parkinson's Disease LRRK2 kinase to block primary cilia and Sonic hedgehog signaling in the brain. Elife, e40202.
303771711:100Kulkarni, S.S., et al. 2018. WDR5 regulates left-right patterning via chromatin-dependent and -independent functions. Development, dev159889.
301314411:50 (supe)Kurtulmus, B., et al. 2018. LRRC45 contributes to early steps of axoneme extension. Journal of Cellular Science, jcs223594.
30100262not listedGalati, D.F., et al. 2018. Trisomy 21 Represses Cilia Formation and Function. Developmental Cell, 641-650.
29615496not listedBeyer, T., et al. 2018. CRISPR/Cas9-mediated Genomic Editing of Cluap1/IFT38 Reveals a New Role in Actin Arrangement. Molecular and Cellular Proteomics, 1285-1294.
29592971not listedRevenkova, E., et al. 2018. The Joubert syndrome protein ARL13B binds tubulin to maintain uniform distribution of proteins along the ciliary membrane. Journal of Cellular Science, jcs212324.
Immunocytochemistry: Mouse
PMID Dilution Publication
371645421:200Hua, K., et al. 2023. Fixation Methods and Immunolabeling for Cilia Proteins in Ciliary and Extraciliary Locations. Methods in Cell Biology, 43-57.
369671421:200Dupuy, A.M.M., et al. 2023. Using Mammary Organoids to Study Cilia. Methods in Cell Biology, 221-233.
365468371:500Tholen, L.E., et al. 2022. Transcription factor HNF1β Controls a Transcriptional Network Regulating Kidney Cell Structure and Tight Junction Integrity. American Journal of Physiology. Renal Physiology, .
36322399not listedNguyen, A., et al. 2022. TTBK2 controls cilium stability by regulating distinct modules of centrosomal proteins. Molecular Biology of the Cell, .
358995291:1000Lv, B., et al. 2022. Arih2 Regulates Hedgehog Signaling Through Smoothened Ubiquitination and ER-Associated Degradation. Journal of Cell Science, .
353222021:500Li, Q., et al. 2022. Disruption of GMNC-MCIDAS multiciliogenesis program is critical in choroid plexus carcinoma development. Cell death and differentiation, .
349146391:100Zhang, W.W., et al. 2022. Peripheral ablation of type Ⅲ adenylyl cyclase induces hyperalgesia and eliminates KOR-mediated analgesia in mice. JCI Insight, e153191.
347834611:1000Yang, D., et al. 2022. Ciliary Type III Adenylyl Cyclase in the VMH Is Crucial for High-Fat Diet-Induced Obesity Mediated by Autophagy. Advanced Science, e2102568.
344633281:500Mecklenburg, N., et al. 2021. Identification of disease-relevant modulators of the SHH pathway in the developing brain. Development, .
343463131:500Hwang, S., et al. 2021. Ciliary and extraciliary Gpr161 pools repress hedgehog signaling in a tissue-specific manner. Elife, e67121.
340558051:200Paulson, D., et al. 2021. Loss of Primary Cilia Protein IFT20 Dysregulates Lymphatic Vessel Patterning in Development and Inflammation. Frontiers in Cell and Developmental Biology, 672625.
33989527not listedGanga, A.K., et al. 2021. Rab34 GTPase mediates ciliary membrane formation in the intracellular ciliogenesis pathway. Current biology, 2895-2905.
33989524not listedStuck, M.W., et al. 2021. Rab34 is necessary for early stages of intracellular ciliogenesis. Current biology, 2887-2894.
337417211:1000Hsiao, Y.C., et al. 2021. The Transition Zone Protein AHI1 Regulates Neuronal Ciliary Trafficking of MCHR1 and Its Downstream Signaling Pathway. Journal of Neuroscience, 3932-3943.
337272501:2000Dhekne, H.S., et al. 2021. LRRK2-phosphorylated Rab10 sequesters Myosin Va with RILPL2 during ciliogenesis blockade. . Life science alliance, 5.
336539481:2000Sobu, Y., et al. 2021. Pathogenic LRRK2 regulates ciliation probability upstream of tau tubulin kinase 2 via Rab10 and RILPL1 proteins. PNAS: USA, e2005894118.
333720371:1000Barbeito, P., et al. 2020. HTR6 and SSTR3 ciliary targeting relies on both IC3 loops and C-terminal tails. Life Science Alliance, e202000746.
333504961:500La Sala, G., et al. 2020. Gpr37l1/prosaposin receptor regulates Ptch1 trafficking, Shh production, and cell proliferation in cerebellar primary astrocytes. Journal of Neuroscience Research, .
330823191:2000Wei, T., et al. 2020. Siah2 integrates mitogenic and extracellular matrix signals linking neuronal progenitor ciliogenesis with germinal zone occupancy. Nature Communications, 5312.
325312771:1000Ho, E.K., et al. 2020. Transient Primary Cilia Mediate Robust Hedgehog Pathway-Dependent Cell Cycle Control. Current Biology, 2829-2835.
32494665not listedProsseda, P.P., et al. 2020. Optogenetic stimulation of phosphoinositides reveals a critical role of primary cilia in eye pressure regulation. Science Advances, eaay8699.
324357931:1000Desai, P.B., et al. 2020. Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling. Journal of Cellular Biology, e201912104.
321297621:1000Gigante, E.D., et al. 2020. ARL13B regulates Sonic hedgehog signaling from outside primary cilia. Elife, e50434.
317615341:1000Hilgendorf, K.I., et al. 2019. Omega-3 Fatty Acids Activate Ciliary FFAR4 to Control Adipogenesis. Cell, 1289-1305.
313912391:2000Muñoz-Estrada, J., et al. 2019. Ahi1 promotes Arl13b ciliary recruitment, regulates Arl13b stability and is required for normal cell migration. Journal of Cellular Science, jcs230680.
313905721:50Uzquiano, A., et al. 2019. Mutations in the Heterotopia Gene Eml1/EML1 Severely Disrupt the Formation of Primary Cilia. Cell Reports, 1596-1611.
313884141:300Lauring, M.C., et al. 2019. New software for automated cilia detection in cells (ACDC). Cilia, 1.
312495121:300Bansal, R., et al. 2019. Hedgehog Pathway Activation Alters Ciliary Signaling in Primary Hypothalamic Cultures. Frontiers in Cellular Neuroscience, 266.
31186137not listedWang, B., et al. 2019. Cilia function is associated with axon initial segment morphology. Biochemical and Biophysical Research Communications, 15-21.
310686771:500Wildung, M., et al. 2019. Transcription factor TAp73 and microRNA-449 complement each other to support multiciliogenesis. Cell Death and Differentiation, 2740-2757.
309056051:200Engelke, M.F., et al. 2019. Acute Inhibition of Heterotrimeric Kinesin-2 Function Reveals Mechanisms of Intraflagellar Transport in Mammalian Cilia. Current Biology, 1137-1148.
30478281not listedKilander, M.B., et al. 2018. A rare human CEP290 variant disrupts the molecular integrity of the primary cilium and impairs Sonic Hedgehog machinery. Scientific Reports, 17335.
304638521:500Hsiao, C.J., et al. 2018. Gli2 modulates cell cycle re-entry through autophagy-mediated regulation of the length of primary cilia. Journal of Cellular Science, jcs221218.
304107311:1000Hoang-Minh, L.B., et al. 2018. Glioma cell proliferation is enhanced in the presence of tumor-derived cilia vesicles. Cilia, 6.
303981481:1000Dhekne, H.S., et al. 2018. A pathway for Parkinson’s Disease LRRK2 kinase to block primary cilia and Sonic hedgehog signaling in the brain. Elife, 7, p.e40202.. Elife, e40202.
299459041:2000Drummond, M.L., et al. 2018. Actin polymerization controls cilia-mediated signaling. Journal of Cellular Biology, 3255-3266.
296789091:1000Lesiak, A.J., et al. 2018. Restoration of Physiological Expression of 5-HT 6 Receptor into the Primary Cilia of Null Mutant Neurons Lengthens Both Primary Cilia and Dendrites. Molecular Pharmacology, 731-742.
295716131:500Gigante, E.D., et al. 2018. Hypomorphic Smo mutant with inefficient ciliary enrichment disrupts the highest level of vertebrate Hedgehog response. Developmental Biology, 152-162.
295405311:1000Prinz, E., et al. 2018. Intraflagellar transport is deeply integrated in hedgehog signaling. Molecular Biology of the Cell, 1178-1189.
28931427not listedHu, L., et al. 2017. Serotonin 5-HT6 receptors affect cognition in a mouse model of Alzheimer's disease by regulating cilia function. Alzheimers Research and Therapy, 76.
286110831:400Hassounah, N.B., et al. 2017. Inhibition of Ciliogenesis Promotes Hedgehog Signaling, Tumorigenesis, and Metastasis in Breast Cancer. Molecular Cancer Research, 1421-1430.
285306761:300Lu, H., et al. 2017. Mutations in DZIP1L, which encodes a ciliary-transition-zone protein, cause autosomal recessive polycystic kidney disease. Nature Genetics, 1025-1034.
28459465not listedYang, N., et al. 2017. INTU is essential for oncogenic Hh signaling through regulating primary cilia formation in basal cell carcinoma. Oncogene, 4997-5005.
28410364not listedPearring, J.N., et al. 2017. Loss of Arf4 causes severe degeneration of the exocrine pancreas but not cystic kidney disease or retinal degeneration. PLoS Genetics, e1006740.
283524621:200Hua, K., et al. 2017. Fixation methods can differentially affect ciliary protein immunolabeling.. Cilia., 5.
28154160not listedBadgandi, H.B., et al. 2017. Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins.. J Cell Biol., 743-760.
Immunocytochemistry: Rat
PMID Dilution Publication
346583371:1000Khan, S.S., et al. 2021. Pathogenic LRRK2 control of primary cilia and Hedgehog signaling in neurons and astrocytes of mouse brain. Elife, e67900.
336509691:1000Tereshko, L., et al. 2021. Ciliary neuropeptidergic signaling dynamically regulates excitatory synapses in postnatal neocortical pyramidal neurons. Elife, e65427.
Immunocytochemistry: Zebrafish
PMID Dilution Publication
339323381:1000Truong, M.E., et al. 2021. Vertebrate cells differentially interpret ciliary and extraciliary cAMP. Cell, 2911-2926.
Immunohistochemistry: Human
PMID Dilution Publication
372115721:2000Ning, K, et al. 2023. Cilia-associated wound repair mediated by IFT88 in retinal pigment epithelium. Scientific reports, 8205.
36580912not listedWu, C.T., et al. 2022. SARS-CoV-2 Replication in Airway Epithelia Requires Motile Cilia and Microvillar Reprogramming. Cell, .
344962391:500Goranci-Buzhala, G., et al. 2021. Cilium induction triggers differentiation of glioma stem cells. Cell Reports, 109656.
336199091:400Dang, L.T., et al. 2021. STRADA-mutant human cortical organoids model megalencephaly and exhibit delayed neuronal differentiation. Developmental Neurobiology, 696-709.
33306870not listedNing, K., et al. 2020. Defective INPP5E distribution in NPHP1-related Senior-Loken syndrome. Molecular Genetics and Genomic Medicine, e1566.
Immunohistochemistry: Mouse
PMID Dilution Publication
372115721:2000Ning, K, et al. 2023. Cilia-associated Wound Repair Mediated by IFT88 in Retinal Pigment Epithelium. Scientific Reports, 8205.
366933201:1000Zhang, K., et al. 2023. Primary Cilia are WNT-transducing Organelles Whose Biogenesis is Controlled by a WNT-PP1 Axis. Developmental Cell, 139-154.
366613571:500Habif, J.C., et al. 2023. The Role of a Ciliary GTPase in the Regulation of Neuronal Maturation of Olfactory Sensory Neurons. Development, .
366561181:500Jewett, C.E., et al. 2023. Trisomy 21 Induces Pericentrosomal Crowding Delaying Primary Ciliogenesis and Mouse Cerebellar Development. eLife, .
364962001:1000Dupuy, V., et al. 2023. Spatiotemporal Dynamics of 5-HT6 Receptor Ciliary Localization During Mouse Brain Development. Neurobiology of Disease, 105949.
34057457not listedDuclos, M., et al. 2021. Three-Dimensional Imaging Of Organoids To Study Primary Ciliogenesis During Ex Vivo Organogenesis. Journal Of Visualized Experiments, .
358061431:200Suiwal, S., et al. 2022. Ciliary Proteins Repurposed by the Synaptic Ribbon: Trafficking Myristoylated Proteins at Rod Photoreceptor Synapses. International Journal of Molecular Sciences, 7135.
353222021:500Li, Q., et al. 2022. Disruption of GMNC-MCIDAS multiciliogenesis program is critical in choroid plexus carcinoma development. Cell death and differentiation, .
347055061:100Wilson, M.M., et al. 2021. An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis. Science Advances, eabf6063.
346106371:500Lo, H.F., et al. 2021. Δ9-Tetrahydrocannabinol inhibits Hedgehog-dependent patterning during development. Development, .
344923101:500Boschen, K.E., et al. 2021. Prenatal alcohol exposure disrupts Sonic hedgehog pathway and primary cilia genes in the mouse neural tube. Reproductive Toxicology, 136-147.
34366878not listedNingappa, M., et al. 2021. Mechanisms of Impaired Lung Development and Ciliation in Mannosidase-1-Alpha-2 ( Man1a2) Mutants. Frontiers in Physiology, 658518.
341327781:500Suciu, S.K., et al. 2021. Smoothened and ARL13B are critical in mouse for superior cerebellar peduncle targeting. Genetics, iyab084.
34057457not listedDuclos, M., et al. 2021. Three-Dimensional Imaging of Organoids to Study Primary Ciliogenesis During ex vivo Organogenesis. Journal of Visualized Experiments, .
340558051:4000Paulson, D., et al. 2021. Loss of Primary Cilia Protein IFT20 Dysregulates Lymphatic Vessel Patterning in Development and Inflammation. Frontiers in Cell and Developmental Biology, 672625.
337417211:1000Hsiao, Y.C., et al. 2021. The Transition Zone Protein AHI1 Regulates Neuronal Ciliary Trafficking of MCHR1 and Its Downstream Signaling Pathway. Journal of Neuroscience, 3932-3943.
337113421:200Sharif, A.S., et al. 2021. Deletion of the phosphatase INPP5E in the murine retina impairs photoreceptor axoneme formation and prevents disc morphogenesis. Journal of Biological Chemistry, 100529.
331754361:500Ramos, C., et al. 2021. Neuron-specific cilia loss differentially alters locomotor responses to amphetamine in mice. Journal of Neuroscience Research, 827-842.
331037371:500Diaz, J., et al. 2020. YIF1B mutations cause a post-natal neurodevelopmental syndrome associated with Golgi and primary cilium alterations. Brain, 2911-2928.
330823191:2000Wei, T., et al. 2020. Siah2 integrates mitogenic and extracellular matrix signals linking neuronal progenitor ciliogenesis with germinal zone occupancy. Nature Communications, 5312.
330465071:5 (supe)Casoni, F., et al. 2020. ZFP423 regulates early patterning and multiciliogenesis in the hindbrain choroid plexus. Development, dev190173.
32938678not listedDias, J.M., et al. 2020. A Shh/Gli-driven three-node timer motif controls temporal identity and fate of neural stem cells. Science Advances, eaba8196.
329175881:1000Stoufflet, J., et al. 2020. Primary cilium-dependent cAMP/PKA signaling at the centrosome regulates neuronal migration. Science Advances, eaba3992.
328402121:1500, et al. 2020. A transient role of the ciliary gene Inpp5e in controlling direct versus indirect neurogenesis in cortical development. Elife, e58162.
327022911:500Somatilaka, B.N., et al. 2020. Ankmy2 Prevents Smoothened-Independent Hyperactivation of the Hedgehog Pathway via Cilia-Regulated Adenylyl Cyclase Signaling. Developmental Cell, 710-726.
321695531:50Fiore, L., et al. 2020. Optic vesicle morphogenesis requires primary cilia. Developmental Biology, 119-128.
321297621:1000Gigante, E.D., et al. 2020. ARL13B regulates Sonic hedgehog signaling from outside primary cilia. Elife, e50434.
321223601:250Sterpka, A., et al. 2020. Diverged morphology changes of astrocytic and neuronal primary cilia under reactive insults. Molecular Brain, 28.
319647741:2000Constable, S., et al. 2020. The ciliary phosphatidylinositol phosphatase Inpp5e plays positive and negative regulatory roles in Shh signaling. Development, dev183301.
319348641:500Bowie, E., et al. 2020. TTBK2 and primary cilia are essential for the connectivity and survival of cerebellar Purkinje neurons. Elife, e51166.
31887266not listedShylo, N.A., et al. 2020. Loss of ciliary transition zone protein TMEM107 leads to heterotaxy in mice. Developmental Biology, 187-199.
316907471:500Fish, E.W., et al. 2019. Cannabinoids Exacerbate Alcohol Teratogenesis by a CB1-Hedgehog Interaction. Scientific Reports, 16057.
316856501:1000Matsumoto, M., et al. 2019. Dynamic Changes in Ultrastructure of the Primary Cilium in Migrating Neuroblasts in the Postnatal Brain. Journal of Neuroscience, 9967-9988.
313218791:200Freeman, S., et al. 2019. Proteostasis is essential during cochlear development for neuron survival and hair cell polarity. EMBO Reports, e47097.
309041801:500Gigante, E.D., et al. 2019. Corrigendum to "Hypomorphic Smo mutant with inefficient ciliary enrichment disrupts the highest level of vertebrate Hedgehog response" [Develop. Biol. 437 (2018) 152-162]. Developmental Biology, 63.
307992401:500Legué, E., et al. 2019. Tulp3 Is a Ciliary Trafficking Gene that Regulates Polycystic Kidney Disease. Current Biology, 803-812.
307992391:500Hwang, S.H., et al. 2019. Tulp3 Regulates Renal Cystogenesis by Trafficking of Cystoproteins to Cilia. Current Biology, 790-802.
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306922211:1500Andreu-Cervera, A., et al. 2019. The Ciliopathy Gene Ftm/Rpgrip1l Controls Mouse Forebrain Patterning via Region-Specific Modulation of Hedgehog/Gli Signaling. Journal of Neuroscience, 2398-2415.
293422441:500Chen, J.L., et al. 2019. Gli2 Rescues Delays in Brain Development Induced by Kif3a Dysfunction. Cerebral Cortex, 751-764.
30157434not listedHaag, N., et al. 2018. The Actin Nucleator Cobl Is Critical for Centriolar Positioning, Postnatal Planar Cell Polarity Refinement, and Function of the Cochlea. Cell Reports, 2418-2431.
29626631not listedDesai, P.B., et al. 2018. Ift25 is not a cystic kidney disease gene but is required for early steps of kidney development. Mechanisms of Development, 44851.
Immunohistochemistry: Non-Human Primate
PMID Dilution Publication
316856501:1000Matsumoto, M., et al. 2019. Dynamic Changes in Ultrastructure of the Primary Cilium in Migrating Neuroblasts in the Postnatal Brain. Journal of Neuroscience, 9967-9988.
Immunohistochemistry: Rat
PMID Dilution Publication
318258201:2000Ferent, J., et al. 2019. The Ciliary Protein Arl13b Functions Outside of the Primary Cilium in Shh-Mediated Axon Guidance. Cell Reports, 3356-3366.
Immunohistochemistry: Xenopus
PMID Dilution Publication
371726411:100Deniz, E, et al. 2023. CFAP45, a Heterotaxy and Congenital Heart Disease Gene, Affects Cilia Stability. Developmental Biology, 75-88.
Immunohistochemistry: Zebrafish
PMID Dilution Publication
315035511:150Arribat, Y., et al. 2019. Sonic Hedgehog repression underlies gigaxonin mutation-induced motor deficits in giant axonal neuropathy. Journal of Clinical Investigation, 5312-5326.
306838961:300Insinna, C., et al. 2019. Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport. Nature Communications, 428.
Western Blot: Human
PMID Dilution Publication
351479771:1000Huang, M., et al. 2022. Cell cycle arrest induced by trichoplein depletion is independent of cilia assembly. Journal of cellular physiology, .
310237191:500Odabasi, E., et al. 2019. Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation. EMBO Reports, e47723.
Western Blot: Mouse
PMID Dilution Publication
366613571:500Habif, J.C., et al. 2023. The Role of a Ciliary GTPase in the Regulation of Neuronal Maturation of Olfactory Sensory Neurons. Development, .
352597521:1000Hamada, N., et al. 2022. Expression Analyses of Cep152, a Responsible Gene Product for Autosomal Recessive Primary Microcephaly, During Mouse Brain Development. Developmental Neuroscience, 162-170.
337417211:1000Hsiao, Y.C., et al. 2021. The Transition Zone Protein AHI1 Regulates Neuronal Ciliary Trafficking of MCHR1 and Its Downstream Signaling Pathway. Journal of Neuroscience, 3932-3943.
321297621:1000Gigante, E.D., et al. 2020. ARL13B regulates Sonic hedgehog signaling from outside primary cilia. Elife, e50434.
30799240not listedLegué, E., et al. 2019. Tulp3 Is a Ciliary Trafficking Gene that Regulates Polycystic Kidney Disease. Current Biology, 803-812.
28931427not listedHu, L., et al. 2017. Serotonin 5-HT6 receptors affect cognition in a mouse model of Alzheimer's disease by regulating cilia function. Alzheimers Research and Therapy, 76.
28576874not listedMonis, W.J., et al. 2017. BLOC-1 is required for selective membrane protein trafficking from endosomes to primary cilia. Journal of Cell Biology, 2131-2150.
Additional Publications: Unspecified
PMID Publication
33531668Luo, M., et al. 2021. Disrupted intraflagellar transport due to IFT74 variants causes Joubert syndrome. Genetics in Medicine, 1041-1049.
29257953Sigg, M.A., et al. 2017. Evolutionary Proteomics Uncovers Ancient Associations of Cilia with Signaling Pathways. Developmental Cell, 744-762.
28719464Coy, S., et al. 2017. Nuclear CRX and FOXJ1 Expression Differentiates Non-Germ Cell Pineal Region Tumors and Supports the Ependymal Differentiation of Papillary Tumor of the Pineal Region. The American Journal of Surgical Pathology, 1410-1421.
28448009Shimada, I.S., et al. 2017. Using Primary Neurosphere Cultures to Study Primary Cilia. Journal of Visualized Experiments, 55315.
28017328Nager, A.R., et al. 2017. An Actin Network Dispatches Ciliary GPCRs into Extracellular Vesicles to Modulate Signaling. Cell, 252-263.
27799542Moore, B.S., et al. 2016. Cilia have high cAMP levels that are inhibited by Sonic Hedgehog-regulated calcium dynamics. PNAS: USA, 13069-13074.
27562488Coy, S., et al. 2016. Distinct patterns of primary and motile cilia in Rathke's cleft cysts and craniopharyngioma subtypes. Modern Pathology, 1446-1459.
27493724Kukic, I., et al. 2016. The IN/OUT assay: a new tool to study ciliogenesis. Cilia, 23.
27261005Ha, S., et al. 2016. Mutations in Dnaaf1 and Lrrc48 Cause Hydrocephalus, Laterality Defects, and Sinusitis in Mice. G3, 2479-2487.
27094867Cortés, C.R., et al. 2016. Mutations in human C2CD3 cause skeletal dysplasia and provide new insights into phenotypic and cellular consequences of altered C2CD3 function. Scientific Reports, 24083.
27046536Emechebe, U., et al. 2016. T-box3 is a ciliary protein and regulates stability of the Gli3 transcription factor to control digit number. Elife, e07897.
27002170Pal, K., et al. 2016. Smoothened determines β-arrestin-mediated removal of the G protein-coupled receptor Gpr161 from the primary cilium. Journal of Cell Biology, 861-875.
26999738Li, L., et al. 2016. Sonic Hedgehog promotes proliferation of Notch-dependent monociliated choroid plexus tumour cells. Nature Cell Biology, 418-430.
26884322Bhattacharyya, S., et al. 2016. Endocytic recycling protein EHD1 regulates primary cilia morphogenesis and SHH signaling during neural tube development.. Sci Rep., 20727.
26859289Stewart, K., et al. 2016. A Point Mutation in p190A RhoGAP Affects Ciliogenesis and Leads to Glomerulocystic Kidney Defects.. PLoS Genet., e1005785.
26658865Yao, P.J., et al. 2015. Dendrosomatic Sonic Hedgehog Signaling in Hippocampal Neurons Regulates Axon Elongation.. J Neurosci., 16126-16141.
26493400Endicott, S.J., et al. 2015. The NIMA-like kinase Nek2 is a key switch balancing cilia biogenesis and resorption in the development of left-right asymmetry.. Development., 4068-4079.
26446227Joiner, A.M., et al. 2015. Primary Cilia on Horizontal Basal Cells Regulate Regeneration of the Olfactory Epithelium.. J Neurosci., 13761-13772.
26305592Garcia-Gonzalo, F.R., et al. 2015. Phosphoinositides Regulate Ciliary Protein Trafficking to Modulate Hedgehog Signaling.. Dev Cell., 400-409.
26074404Kowal, T.J., et al. 2015. Primary cilia found on HeLa and other cancer cells.. Cell Biol Int., 1341-1347.
26071364Laclef, C., et al. 2015. The role of primary cilia in corpus callosum formation is mediated by production of the Gli3 repressor.. Hum Mol Genet., 4997-5014.
26053317Omori, Y., et al. 2015. Identification of G Protein-Coupled Receptors (GPCRs) in Primary Cilia and Their Possible Involvement in Body Weight Control.. PLoS One., e0128422.

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