Anti-Arginase I (ARG1) Antibody

Name: Anti-Arginase I (ARG1) Antibody
Brand: PhosphoSolutions
SKU: 146-ARG1
Price: 456.0 USD
Availability: InStock

Our Anti-Arginase I (ARG1) primary antibody from PhosphoSolutions is chicken polyclonal. It detects guinea pig, human, mouse, and rat Arginase I (ARG1) and is total IgY fraction. It is great for use in WB, IHC.

Immunofluorescent image of mouse retina specifically labeling arginase (Cat. 146-ARG, 1:500, green) and CD206 (red). The blue nuclear stain is DAPI. The image was kindly provided by Sarah Gough, University of Miami Miller School of Medicine.

Western blot of mouse liver lysate showing specific immunolabeling of the ~35, 38 kDa arginase I protein.

Immunofluorescent image of mouse retina specifically labeling arginase (Cat. 146-ARG, 1:500, green) and CD206 (red). The blue nuclear stain is DAPI. The image was kindly provided by Sarah Gough, University of Miami Miller School of Medicine.

Click on image to zoom

Species Reactivity

Guinea Pig, Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host Species

Chicken

Go to Full Product Details

Data Sheet

Trial Size

SKU: 146-ARG1

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Arginase I (ARG1)

Scientific Background

Arginase I, a cytosolic enzyme, is highly expressed in the liver and is a component of the urea cycle (Jenkinson et al, 1996). There are two isoforms of arginase, both catalyze the conversion of arginine to ornithine and urea, but differ based on subcellular localization, tissue distribution, and enzymatic properties (Jenkinson et al, 1996). Arginase I has been identified in macrophages in response to various pathogen exposures causing an upregulation of inducible nitric oxide synthase, iNOS, yielding increased levels of nitric oxide (Morris et al, 1998) and inflammation (Poljakovic et al, 2007).

Format

Total IgY fraction

Clonality

Polyclonal

Isotype

IgY

Applications

ELISA, IHC, WB

Host Species

Chicken

Gene Name

ARG1

Molecular Weight

35/38 kDa

Immunogen

Full-length recombinant rat liver arginase I expressed in and purified from E. Coli.

Immunogen Species

Rat

Species Reactivity

Guinea Pig, Human, Mouse, Rat

Antibody Registry ID

AB_2732850

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Total IgY fraction.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
IHC: 1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~35-38 kDa arginase I protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

A I; Al; ARG 1; arg1; ARGI1_HUMAN; Arginase 1; Arginase liver; Arginase type I; Arginase-1; Arginase1; Liver type arginase; Liver-type arginase; Type I arginase

UniProt ID

P07824
UniProt Summary: Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. {ECO:0000305}.; Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed:15546957, PubMed:16709924, PubMed:19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure. {ECO:0000250|UniProtKB:Q61176, ECO:0000269|PubMed:15546957, ECO:0000269|PubMed:16709924, ECO:0000269|PubMed:19380772}.

NCBI Gene ID

29221

Shipping

Blue Ice

Western Blot Protocol: Download

Recover password

Create my account

Anti-Arginase I (ARG1) Antibody

SKU: 146-ARG1

Product Details

Bulk Order Anti-Arginase I (ARG1) Antibody

Related Products

Recently Viewed