Our Annexin A1 mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Annexin A1 and is Protein G purified. It is great for use in WB, ICC, IP.
Western blot of human A431 cells (lane 1), A549 cells (lane 2), LNCaP cells (lane 3), full-length recombinant human annexin A1 (lane 4), and recombinant human annexin A2 (lane 5). The blot was probed with mouse monoclonal anti-Annexin A1 antibody (AM0211) at 1:1000 (lanes 1-5).
The Annexin family is composed of at least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. Annexins have roles in membrane fusion, endocytosis, secretion, and repair. Annexin A1 binds to cellular membranes in a calcium-dependent manner, promotes membrane fusion and endocytosis, and has been implicated as an anti-inflammatory mediator. Annexin A2 is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of corticosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. Annexin A5 is a PKC inhibitor, directly interacts with VEGFR2 receptor, and binds phosphatidylserine to inhibit blood coagulation. Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras.
Protein G Purified
Monoclonal
M021
IgG1
ICC, IP, WB
Mouse
ANXA1
38
Clone M021 was generated from a proprietary antigen related to human Annexin A1 in the A549 lung carcinoma cell line.
Human
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:100
IP: 1:100
Unconjugated
Clone M021 detects a 38 kDa* protein corresponding to the molecular mass of Annexin A1 on SDS-PAGE immunoblots of human A431, MDA-MB-231, and A549. The antibody also detects full length recombinant human Annexin A1, but not Annexin A2 or Annexin A5 recombinant proteins. The antibody works for western blot, ELISA, immunocytochemical labeling, and immunoprecipitation.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I1, Phospholipase A2 inhibitory protein p35, ANXA1, ANX1, LPC1
Vaňková, K, et al. 2018. Chlorophyll-Mediated Changes in the Redox Status of Pancreatic Cancer Cells Are Associated with Its Anticancer Effects. Oxidative medicine and cellular longevity, 4069167.
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