Our Anti-Amyloid Precursor Protein rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Amyloid Precursor Protein and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~115 kDa APP protein.
A large body of evidence has implicated the amyloid precursor protein (APP) in the pathogenesis of Alzheimer's disease (AD) (Kamenetz et al., 2003). The phosphorylation of APP at Thr668 is thought to play a critical role in generation of the soluble APP (beta) and beta-amyloid peptide (abeta) which are the major components of senile plaques in patient brains inflicted with AD (Liu et al., 2003; Ando et al., 2001).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
APP
115 kDa
Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat APP, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
Canine, Chicken, Human, Non-Human Primate
AB_2492039
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from rabbit serum by affinity purification on a column made with the C-terminal peptide used as antigen.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~115 kDa APP protein. Immunolabeling of the APP protein band is completely blocked by preadsorption of the antibody with the peptide used as antigen.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Ando K, Iijima KI, Elliott JI, Kirino Y, Suzuki T (2001) Phosphorylation-dependent regulation of the interaction of amyloid precursor protein with Fe65 affects the production of beta-amyloid. J Biol Chem 276:40353-40361. PMID: 11517218
Kamenetz, F., Tomita, T., Hsieh, H., Seabrook, G., Borchelt, D., Iwatsubo, T., Sisodia, S. and Malinow, R., 2003. APP processing and synaptic function. Neuron, 37(6), pp.925-937. PMID: 12670422
Liu F, Su Y, Li B, Zhou Y, Ryder J, Gonzalez-DeWhitt P, May PC, Ni B (2003) Regulation of amyloid precursor protein (APP) phosphorylation and processing by p35/cdk5 and p25/cdk5. FEBS Lett 547:193-196. PMID: 12860412
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