Immunoblot versus RBM and lysates of WT and AMIGO-1 KO mouse brains probed with L86/33 (left), K89/34 (middle) and N52A/42 (right) TC supe. Mouse brain samples courtesy of Juha Kuja-Panula (University of Helsinki, Finland).
Amphoterin-induced protein 1 (AMIGO-1) (Alivin-2)
Amphoterin-induced protein 1, amphoterin-induced gene and open reading frame, Adhesion Molecule With Ig Like Domain 1 or Amigo-1 is encoded by the gene AMIGO1 and is a member of the AMIGO family. Amigo1 acts as a neuronal adhesion protein and contains leucine-rich repeat and immunoglobin domains. It is thought to play a role in cell to cell interactions and in neuronal plasticity/regenereation. Amigo1 is expressed in the nervous system and at low levels in liver, kidney, spleen, lung and heart.
IHC, ICC, WB
Amigo1 Ali2 Amigo Kiaa1163
60-80 kDa depending on maturity/glycosylation
Fusion protein amino acids 554-574 (cytoplasmic C-terminus) of human AMIGO-1 (also known as Amphoterin-induced protein 1, Alivin-2, Ali2, AMIGO and KIAA1163, accession number Q86WK6)
Rat: 100% identity (99/99 amino acids identical)
Mouse: 100% identity (99/99 amino acids identical)
Less than 40% identity with AMIGO-2 and AMIGO-3
Human, Mouse, Rat
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.