Anti-Alpha synuclein Antibody

Our Anti-Alpha synuclein chicken polyclonal primary antibody detects bovine, chicken, horse, human, mouse, pig, and rat Alpha synuclein, and is affinity-purified. It is validated for use in ICC, IHC-Frozen, WB.

Left: Rat cerebellum section stained with chicken anti-α-synuclein antibody (red, 1:3,000) by Immunohistochemistry. Section was co-stained with rabbit anti-GFAP (green, R-1374-50, 1:5,000) and DAPI nuclear dye (blue). IHC method: Following transcardial perfusion with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut into 45 uM sections, and free-floating sections were stained. The α-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells. Right: Western blot analysis of tissue lysates. Lane 1: MWM; Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord. Antibody dilution: 1:2,000. A strong band at about 15 kDa corresponds to the α-synuclein protein in brain extracts, which are rich in synapses.
Left: Analysis of alpha-synuclein expression in mixed rat neuron-glial cultures by Immunocytochemistry. The alpha-synuclein antibody (red) stains vesicular structures the perikarya and processes of the neurons. The antibody reveals that some of the neuronal perikarya contain more alpha-synuclein than others. Green: MAP2, Blue: DNA. Right: Detection of alpha-synuclein monomer (~15 kDa) in crude rat brain extract by Western Blotting.
Left: Rat cerebellum section stained with chicken anti-α-synuclein antibody (red, 1:3,000) by Immunohistochemistry. Section was co-stained with rabbit anti-GFAP (green, R-1374-50, 1:5,000) and DAPI nuclear dye (blue). IHC method: Following transcardial perfusion with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut into 45 uM sections, and free-floating sections were stained. The α-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells. Right: Western blot analysis of tissue lysates. Lane 1: MWM; Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord. Antibody dilution: 1:2,000. A strong band at about 15 kDa corresponds to the α-synuclein protein in brain extracts, which are rich in synapses.
Left: Rat cerebellum section stained with chicken anti-α-synuclein antibody (red, 1:3,000) by Immunohistochemistry. Section was co-stained with rabbit anti-GFAP (green, R-1374-50, 1:5,000) and DAPI nuclear dye (blue). IHC method: Following transcardial perfusion with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut into 45 uM sections, and free-floating sections were stained. The α-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells. Right: Western blot analysis of tissue lysates. Lane 1: MWM; Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord. Antibody dilution: 1:2,000. A strong band at about 15 kDa corresponds to the α-synuclein protein in brain extracts, which are rich in synapses.
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Bovine, Chicken, Horse, Human, Mouse, Pig, Rat
ICC, IHC, WB
Chicken

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Data Sheet

SKU: C-1795-50

Volume/Size: 50 µg
Price:
$327.00

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