Anti-Alpha synuclein Antibody

Name: Anti-Alpha synuclein Antibody
Brand: Biosensis
SKU: C-1795-50
Price: 337.0 USD
Availability: InStock

Our Anti-Alpha synuclein chicken polyclonal primary antibody detects bovine, chicken, horse, human, mouse, pig, and rat Alpha synuclein, and is affinity-purified. It is validated for use in ICC, IHC-Frozen, WB.

Left: Rat cerebellum section stained with chicken anti-α-synuclein antibody (red, 1:3,000) by Immunohistochemistry. Section was co-stained with rabbit anti-GFAP (green, R-1374-50, 1:5,000) and DAPI nuclear dye (blue). IHC method: Following transcardial perfusion with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut into 45 uM sections, and free-floating sections were stained. The α-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells. Right: Western blot analysis of tissue lysates. Lane 1: MWM; Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord. Antibody dilution: 1:2,000. A strong band at about 15 kDa corresponds to the α-synuclein protein in brain extracts, which are rich in synapses.

Left: Analysis of alpha-synuclein expression in mixed rat neuron-glial cultures by Immunocytochemistry. The alpha-synuclein antibody (red) stains vesicular structures the perikarya and processes of the neurons. The antibody reveals that some of the neuronal perikarya contain more alpha-synuclein than others. Green: MAP2, Blue: DNA. Right: Detection of alpha-synuclein monomer (~15 kDa) in crude rat brain extract by Western Blotting.

Left: Rat cerebellum section stained with chicken anti-α-synuclein antibody (red, 1:3,000) by Immunohistochemistry. Section was co-stained with rabbit anti-GFAP (green, R-1374-50, 1:5,000) and DAPI nuclear dye (blue). IHC method: Following transcardial perfusion with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut into 45 uM sections, and free-floating sections were stained. The α-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells. Right: Western blot analysis of tissue lysates. Lane 1: MWM; Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord. Antibody dilution: 1:2,000. A strong band at about 15 kDa corresponds to the α-synuclein protein in brain extracts, which are rich in synapses.

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Species Reactivity

Bovine, Chicken, Horse, Human, Mouse, Pig, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

SKU: C-1795-50

Volume/Size: 50 µg

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Alpha synuclein

Scientific Background

May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. Ref: uniprot.org.

Format

Affinity-purified

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Immunogen

Full length human protein with the epitope from amino acids 61-95

Immunogen Species

Human

Species Reactivity

Bovine, Chicken, Horse, Human, Mouse, Pig, Rat

Storage

Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.

Physical State

Liquid

Buffer

PBS, pH 7.4, containing 0.02% sodium azide as preservative.

Dilution Ranges

WB: 1:1000-1:2000
IHC: 1:1000-1:2000
ICC: 1:1000-1:2000

Conjugate

Unconjugated

Specificity

Reacts with human, horse, cow, pig, chicken, rat, mouse.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Alpha synuclein; Non-A beta component of AD amyloid; Alpha-synuclein; Non-A4 component of amyloid precursor; Alpha-synuclein; NACP; isoform NACP140; SNCA; alphaSYN; PARK1; MGC105443; MGC110988; MGC127560; MGC64356; NACP; Non A beta component of AD amyloid; Non A4 component of amyloid; Non A4 component of amyloid precursor; Non-A beta component of AD amyloid; Non-A-beta component of alzheimers disease amyloid; precursor of; Non-A4 component of amyloid precursor; Non-A4 component of amyloid; OTTHUMP00000218549; OTTHUMP00000218551; OTTHUMP00000218552; OTTHUMP00000218553; OTTHUMP00000218554; PARK 1; PARK 4; PARK1; PARK4; Parkinson disease (autosomal dominant Lewy body 4; Parkinson disease familial 1; SNCA; Snca synuclein alpha (non A4 component of amyloid precursor; SYN; Synuclein alpha; Synuclein alpha 140; Synuclein alpha (non A4 component of amyloid precursor; SYUA_HUMAN

UniProt ID

P37840
UniProt Summary: Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity.

NCBI Gene ID

6622

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Anti-Alpha synuclein Antibody

SKU: C-1795-50

Product Details

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