Detect endogenous levels of alpha-synuclein (SNCA), also known as PARK1, NACP, PARK4, by Western Blot, immunohistochemistry, and immunofluorescence using this knockout-validated mouse anti-alpha-synuclein antibody.
Sagittal section of formalin fixed, paraffin-embedded rat brain showing strong staining of alpha synuclein within the molecular layer of the cerebellum. Image at right shows higher magnification of indicated area or interest. Sections were stained with mouse anti-alpha-synuclein (clone 6113-62) antibody at 1:5000 dilution and detected with anti-mouse HRP.
Anti-Alpha Synuclein Antibody
SKU: 77-521-100
Bulk Order Anti-Alpha Synuclein Antibody
Product Details
Alpha-Synuclein
Alpha-synuclein (SNCA), also known as PARK1, NACP, PARK4, is a member of the synuclein family, which also includes beta- and gamma-synuclein. Alpha-synuclein is a highly conserved protein known to be abundant in neurons and especially presynaptic terminals where it serves multiple roles including regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Functionally, alpha-synuclein has been implicated in synaptic plasticity (Liu 2004) and the assembly of snare complexes (Burre 2010) and it is known to be associated with various neuropathologies including Parkinson's Disease, Lewy Body Dementia, and Alzheimer's Disease, where it forms insoluble protein aggregates. Early studies of amyloid deposits in the brains of Alzheimer's patients revealed two previously uncharacterized peptides in addition to the amyloid beta fragment (Ueda 1993), both of which were found to correspond to alpha-synuclein (SNCA). Later studies confirmed that aggregated alpha-synuclein proteins are present in brain lesions (Lewy bodies) that are hallmarks of neurodegenerative synucleinopathies and that alpha-synuclein likely plays a role in the pathogenesis of Parkinson's disease, Lewy body dementia, and Alzheimer’s disease among other neuropathologies. The SNCA antibody was raised against full-length human alpha-synuclein and recognizes endogenous levels of alpha-synuclein in brain by Western blot and immunohistochemistry.
Purified by Protein A Chromatography
1 mg/mL
Monoclonal
6113-62
IgG1
ICC, IHC, WB
Mouse
SNCA
18 kDa
Full length human alpha-synuclein (accession number P37840) produced recombinantly in E. coli.
Human, Mouse, Non-Human Primate, Rat
AB_2924735
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot on lysate from rat whole brain.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
