Anti-Alpha Synuclein Antibody (6113-10)

Name: Anti-Alpha Synuclein Antibody
Brand: Antibodies Incorporated
SKU: 77-520-020
Price: 337.0 USD
Availability: InStock

Our mouse Alpha-Synuclein monoclonal antibody from Antibodies Incorporated is produced in-house from clone 6113-10. It detects human, mouse, non-human primate, and rat Alpha-Synuclein and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Sagittal section of formalin fixed, paraffin-embedded rat brain showing strong staining of alpha synuclein within the molecular layer of the cerebellum. Image at right shows higher magnification of indicated area of interest. Sections were stained with anti-alpha-synuclein (clone 6113-10) antibody at 1:5,000 dilution and detected with anti-mouse HRP.

Western blotting of rat or mouse brain homogenate (10 µg/lane) with Antibodies Incorporated anti-alpha-synuclein antibodies at 1:200 dilution and detected with anti-mouse HRP.

Western blotting of A) WT HEK-293T cell lysate or B) alpha-synuclein knockout HEK-293T cell lysate (10 µg/lane) with Antibodies Incorporated mouse anti-alpha-synuclein antibody (clone 6113-10).

Sagittal section of formalin fixed, paraffin-embedded rat brain showing strong staining of alpha synuclein within the molecular layer of the cerebellum. Image at right shows higher magnification of indicated area of interest. Sections were stained with anti-alpha-synuclein (clone 6113-10) antibody at 1:5,000 dilution and detected with anti-mouse HRP.

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Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

Trial Size

SKU: 77-520-020

Volume: 20 µL

Variant

Price:

$137.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Alpha-Synuclein

Scientific Background

Alpha-synuclein (SNCA), also known as PARK1, NACP, PARK4, is a member of the synuclein family, which also includes beta- and gamma-synuclein. Alpha-synuclein is a highly conserved protein known to be abundant in neurons and especially presynaptic terminals where it serves multiple roles including regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Functionally, alpha-synuclein has been implicated in synaptic plasticity (Liu 2004) and the assembly of snare complexes (Burre 2010) and it is known to be associated with various neuropathologies including Parkinson's Disease, Lewy Body Dementia, and Alzheimer's Disease, where it forms insoluble protein aggregates. Early studies of amyloid deposits in the brains of Alzheimer's patients revealed two previously uncharacterized peptides in addition to the amyloid beta fragment (Ueda 1993), both of which were found to correspond to alpha-synuclein (SNCA). Later studies confirmed that aggregated alpha-synuclein proteins are present in brain lesions (Lewy bodies) that are hallmarks of neurodegenerative synucleinopathies and that alpha-synuclein likely plays a role in the pathogenesis of Parkinson's disease, Lewy body dementia, and Alzheimer’s disease among other neuropathologies. The SNCA antibody was raised against full-length human alpha-synuclein and recognizes endogenous levels of alpha-synuclein in brain by Western blot and immunohistochemistry.

Format

Purified by Protein A Chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

6113-10

Isotype

IgG2a

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

SNCA

Molecular Weight

18 kDa

Immunogen

Full length human alpha-synuclein (accession number P37840) produced recombinantly in E. coli.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Antibody Registry ID

AB_2924734

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:200-1:500
WB Brain: 1:200-1:500
IHC: 1:5000
ICC: 1:1000

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot on lysate from rat whole brain.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Alpha synuclein; NACP; Alpha-synuclein; Alpha-synuclein; isoform NACP140; alphaSYN; MGC105443; MGC110988; MGC127560; MGC64356; NACP; Non A beta component of AD amyloid; Non A4 component of amyloid; Non A4 component of amyloid precursor; Non-A beta component of AD amyloid; Non-A-beta component of alzheimers disease amyloid; precursor of; Non-A4 component of amyloid precursor; Non-A4 component of amyloid; OTTHUMP00000218549; OTTHUMP00000218551; OTTHUMP00000218552; OTTHUMP00000218553; OTTHUMP00000218554; PARK 1; PARK 4; PARK1; PARK4; Parkinson disease (autosomal dominant Lewy body 4; Parkinson disease familial 1; SNCA; Snca synuclein alpha (non A4 component of amyloid precursor; SYN; Synuclein alpha; Synuclein alpha 140; Synuclein alpha (non A4 component of amyloid precursor; SYUA_HUMAN; PARK1

UniProt ID

P37840
UniProt Summary: Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity.

NCBI Gene ID

6622

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Anti-Alpha Synuclein Antibody (6113-10)

SKU: 77-520-020

Product Details

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