Our Anti-Alpha II Spectrin primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat Alpha II Spectrin and is serum. It is great for use in WB, IHC, ICC.
Immunofluorescence of cultured neurons and glia cells showing specific labeling of neuronal perikarya, axons and dendrites with anti-alpha-II-spectrin (cat. 99-A2SR, 1:1000, green), specific labeling of intermediate filaments in glial and fibroblastic cells with anti-vimentin (cat. 2105-VIM, 1:5000, red) and nuclear staining with DAPI (blue).
The spectrin family of cytoskeletal proteins is comprised of 2 alpha genes (α1 and α2) and five beta genes (β1-β5). Spectrins have been shown to function as scaffolding proteins in mechanical support of the plasma membrane as well as bind other membrane proteins and lipids (Bennett and Baines 2001). Defects in spectrin genes have been linked to some forms of hereditary spherocytosis, a type of auto-hemolytic anemia which is characterized by spherical red blood cells that are more prone to lysis (Eber and Lux 2004).
Serum
Polyclonal
IgG
ICC, IHC, WB
Rabbit
SPTAN1
240 kDa
Recombinant construct containing the 7th, 8th, 9th repeats of human alpha II spectrin.
Human, Mouse, Rat
AB_2492031
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Specific for endogenous levels of the ~240 kDa alpha II spectrin protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Bennett, V. and Baines, A.J., 2001. Spectrin and ankyrin-based pathways: metazoan inventions for integrating cells into tissues. Physiological Reviews, 81(3), pp.1353-1392. PMID: 15882777
Eber, S. and Lux, S.E., 2004, April. Hereditary spherocytosis—defects in proteins that connect the membrane skeleton to the lipid bilayer. In Seminars in Hematology, 41(2),pp. 118-141. PMID: 15882777
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