Anti-Alpha II Spectrin Antibody (3D7)

Name: Anti-Alpha II Spectrin Antibody
Brand: PhosphoSolutions
SKU: 98-A2SM
Price: 456.0 USD
Availability: InStock

Our Anti-Alpha II Spectrin primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, human, mouse, and rat Alpha II Spectrin and is Protein G purified. It is great for use in WB, IHC, ICC.

Immunofluorescence of a section of rat cerebellum selectively labeling the submembraneous cytoskeleton on neurons and cell bodies and dendrites of Purkinje cells with alpha-II-spectrin (Cat. 98-A2SM, 1:2000, green) and labeling the processes of Bergmann glia and astrocytes with anti-GFAP (Cat. 621-GFAP, 1:5000, red).

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~240 kDa alpha II spectrin protein.

Immunofluorescence of cultured neurons and glia cells showing specific axonal and dendritic labeling with anti-alpha-II-spectrin (Cat. 98-A2SM, 1:500, green), and nuclear staining with DAPI (blue).

Immunofluorescence of a section of rat cerebellum selectively labeling the submembraneous cytoskeleton on neurons and cell bodies and dendrites of Purkinje cells with alpha-II-spectrin (Cat. 98-A2SM, 1:2000, green) and labeling the processes of Bergmann glia and astrocytes with anti-GFAP (Cat. 621-GFAP, 1:5000, red).

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Species Reactivity

Bovine, Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

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Data Sheet

Trial Size

SKU: 98-A2SM

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Alpha II Spectrin

Scientific Background

The spectrin family of cytoskeletal proteins is comprised of 2 alpha genes (α1 and α2) and five beta genes (β1-β5). Spectrins have been shown to function as scaffolding proteins in mechanical support of the plasma membrane as well as bind other membrane proteins and lipids (Bennett and Baines 2001). Defects in spectrin genes have been linked to some forms of hereditary spherocytosis, a type of auto-hemolytic anemia which is characterized by spherical red blood cells that are more prone to lysis (Eber and Lux 2004).

Format

Protein G Purified

Clonality

Monoclonal

Clone

3D7

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

SPTAN1

Molecular Weight

240 kDa

Immunogen

Recombinant contstruct containing the 7th, 8th, 9th repeats of human alpha II spectrin.

Immunogen Species

Human

Species Reactivity

Bovine, Human, Mouse, Rat

Antibody Registry ID

AB_2492030

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Protein G purified culture supernatant.

Buffer

PBS + 50% glycerol and 5 mm NaN3

Dilution Ranges

WB: 1:3000
WB Brain: 1:3000
IHC: 1:500-1:2000
ICC: 1:500-1:2000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~240 kDa alpha II spectrin protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

ALPHAII-SPECTRIN; Alpha-II spectrin; alpha-fodrin; brain; EIEE5; FLJ17738; FLJ44613; Fodrin; Fodrin alpha chain; Fodrin; alpha; NEAS; Non erythrocytic spectrin alpha; non-erythroid alpha chain ,SPECA; Spectrin alpha chain; Spectrin alpha chain brain; Spectrin alpha non erythrocytic 1; Spectrin; Spectrin non erythroid alpha chain; Spectrin; alpha; non-erythrocytic 1 (alpha-fodrin; nonerythroid; alpha subunit; Spna2; SPTA 2; SPTA2; SPTA2_HUMAN; SPTAN 1; SPTAN1; 7738; non-erythroid alpha chain; SPECA; non-erythrocytic 1

UniProt ID

Q13813
UniProt Summary: Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.

NCBI Gene ID

6709

Shipping

Blue Ice

Western Blot Protocol: Download

General References

Bennett, V. and Baines, A.J., 2001. Spectrin and ankyrin-based pathways: metazoan inventions for integrating cells into tissues. Physiological Reviews, 81(3), pp.1353-1392. PMID: 11427698
Eber, S. and Lux, S.E., 2004, April. Hereditary spherocytosis—defects in proteins that connect the membrane skeleton to the lipid bilayer. In Seminars in Hematology, 41(2),pp. 118-141. PMID: 15071790

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Anti-Alpha II Spectrin Antibody (3D7)

SKU: 98-A2SM

Product Details

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