Immunofluorescence of COS cells expressing 6xHis/Myc-tagged human VDAC1. Green = N144/14, red = Rabbit anti-Myc, blue = Hoechst nuclear stain.
6xHIS or His-tag is a commonly used recombinant tag that consists of 6 sequential histidine residues placed at the amino or carboxy terminus of a targeted sequence. 6xHis is useful for purification of proteins or visualization by western blot or immunocytochemistry.
ELISA, ICC, WB
Identified as off-target mAb in screen for anti-ProtocadherinGammaA3 Neuromabs (immunogen was 6xHis fusion protein amino acids 720-804, variable portion of cytoplasmic C-terminus, of mouse ProtocadherinGammaA3). Target identified as 6xHis by ELISA, immunofluorescence and immunoblot against other 6xHis-tagged fusion proteins.
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
No cross-reactivity reported
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
24 months from opening
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