Anti-14-3-3 protein eta Antibody (3G12)

Name: Anti-14-3-3 protein eta Antibody
Brand: Biosensis
SKU: M-1572-100
Price: 327.0 USD
Availability: InStock

Our Anti-14-3-3 protein eta mouse monoclonal primary antibody detects human, mouse, and rat 14-3-3 protein eta, and is IgG. It is validated for use in ICC, IHC-Frozen, WB.

Image shows rat mixed neuron/glial cultures stained with Mouse monoclonal antibody to 14.3.3 protein eta M-1572-100 antibody (red). Neuronal perikarya are very rich in 14.3.3 protein eta which has a diffuse cytoplasmic staining pattern. Blue is a DNA stain.

Left: Analysis of 14.3.3 eta expression in HeLa cells by Immuncytochemistry using mouse antibody to 14.3.3 protein eta (red, 1:1,000). Blue: DAPI nuclear stain. The antibody reveals the diffuse cytoplasmic distribution of 14.3.3 protein with higher concentration in the perinuclear region. Right: Western blot analysis of whole brain lysates (lanes 2,3), and cell lysates (lanes 4-8), using mouse antibody to 14.3.3 eta (green, 1:5,000). [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y, [8] C6 cells. The strong band at 28 kDa corresponds to 14.3.3 eta protein.

Image shows rat mixed neuron/glial cultures stained with Mouse monoclonal antibody to 14.3.3 protein eta M-1572-100 antibody (red). Neuronal perikarya are very rich in 14.3.3 protein eta which has a diffuse cytoplasmic staining pattern. Blue is a DNA stain.

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

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Data Sheet

SKU: M-1572-100

Volume/Size: 100 µg

Variant

Price:

$327.00

Quantity:

Product Details

Target

14-3-3 protein eta

Scientific Background

14.3.3 protein eta or 14.3.3 binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (Ref SwissProt). 14.3.3 protein eta is widely expressed as both homodimers and heterodimers and are concentrated in the nervous system. High concentrations of 14.3.3 protein eta have been linked to Creutzfeld Jacob Disease, Parkinson's Disease and early-onset schizopherenia.

Format

IgG

Clonality

Monoclonal

Clone

3G12

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Mouse

Immunogen

Full length recombinant 14.3.3 protein ETA expressed in and purified from E. coli.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Production Notes

Protein G purified

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Dilution Ranges

WB: 1:1000-1:5000
IHC: 1:500-1:1000
ICC: 1:500-1:1000

Application Details

A suitable control tissue is rat spinal cord or peripheral nerve homogenate.

Conjugate

Unconjugated

Specificity

Human 14-3-3 ETA protein.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

14.3.3 ; Protein AS1; YWHAH; YWHA1; tyrosine 3-monooxygenase; tryptophan 5-monooxygenase activation protein 1;

UniProt ID

Q04917

Shipping

25°C (ambient)

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Anti-14-3-3 protein eta Antibody (3G12)

SKU: M-1572-100

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