Amylo-Glo Amyloid Plaque Stain Reagent with EtBr Counter Stain

Name: Amylo-Glo Amyloid Plaque Stain Reagent with EtBr Counter Stain
Brand: Biosensis
SKU: TR-400-AG
Price: 377.0 USD
Availability: InStock

Amylo-Glo Ready to Dilute Staining reagent is designed to stain amyloid plaques in tissue sections.

Combined Amylo-Glo labeling of amyloid plaques (blue)with ethidium bromide Nissl (cell body) counterstain (red) in the dentate gyrus region of the hippocampus of the AD/Tg mouse.UV light excitation.

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Species Reactivity

All Mammals, Human, Mouse, Rat

Applications

ICC, IHC

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Data Sheet

SKU: TR-400-AG

Volume/Size: 5 mL

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Price:

$377.00

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Product Details

Product Description

The Biosensis AG-400-AG kit utilizes an ethidium bromide counter stain for a quick and effective way to visualize cell nuclei and cell bodies of cells while under UV illumination allowing the assessment of amyloid plaques and cell/tissue positioning as well in one step.

Amylo-Glo Ready to Dilute Staining reagent is designed to stain amyloid plaques in tissue sections. This novel marker has several advantages over other conventional markers such as Thioflavin S and Congo Red because of its unique chemical and spectral properties. (L. Schmued et al. (2012) J.Neuroscience Methods 209:120- 126). Using Amylo-Glo results in a very bright blue UV excitable stain under physiological conditions that will not bleed through when illuminated with other filters. Its brightness makes it ideal for low magnification quantification studies, while its unique excitation/emission profile and mild staining conditions makes it ideal for combination for multiple immunofluorescent labeling studies. Amylo-Glo RTD is compatible with fresh, frozen, and formalin-fixed immunohistochemistry or cytochemistry, and it is particularly good for confocal and multiple labeling because of its high fluorescent intensity and high resistance to photo-bleaching. Moreover because Amylo-Glo fluoresces in the UV channel, double and triple labeling experiments can be performed very easily (see protocol).

Target

Amyloid plaque

Immunogen Species

Species Independent

Specificity

Amyloid plaques both intraneuronal and vascular for A-G, Etbr, nuclei and cell bodies both DNA and RNA label.

Species Reactivity

All Mammals, Human, Mouse, Rat

Applications

ICC, IHC

Application Details

Staining of amyloid plaques in human and animal tissues, see included protocol. EtBr counter stain stains nuclei and cell bodies for easy identification and spatial orientation.

Method of Analysis

Fluorescence

Format

Purified

Physical State

Liquid, ready to dilute

Production Notes

Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of two fluorescent isomers. No amount of starting material was detected.

Synonyms

AmyloGlo

Usage Statement

For research use only.

Country of Origin

United States

Expiration

6 months after date of receipt (unopened vial).

Storage

The stock solution can be stored for up to 6 months at 2-8°C protected from light. No preservatives. Use sterile technique when handling and proper laboratory procedures.

Shipping

Shipped on ice packs

Amyly-Glo Amyloid Plaque Stain Reagent Protocol

1 bottle containing 40 mL of 10X Amylo-Glo RTD (A-G RTD) solution
1 bottle containing 40 mL of 10X A-G RTD Ethidium Bromide (EtBr RTD) solution

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Amylo-Glo Amyloid Plaque Stain Reagent with EtBr Counter Stain

SKU: TR-400-AG

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