Products: Primary Antibodies

Anti-AMIGO-1 (L86A/37)


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PRODUCT OPTIONS

Catalog # Option Concentration Price*
Catalog #
75-329
Option
Purified
Concentration
1 mg/mL
Price
$205.00
Catalog #
73-329
Option
TC Supernatant
Concentration
Lot dependent
Price
$180.00

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Immunogen:

Fusion protein amino acids 395-493 (cytoplasmic C-terminus) of human AMIGO-1 (also known as Amphoterin-induced protein 1, Alivin-2, Ali2, AMIGO and KIAA1163, accession number Q86WK6) Rat: 100% identity (99/99 amino acids identical) Mouse: 100% identity (99/99 amino acids identical) <40% identity with AMIGO-2 and AMIGO-3

Protein Name(s):

Amphoterin-induced protein 1 (AMIGO-1) (Alivin-2)

Gene Name(s):

Amigo1 Ali2 Amigo Kiaa1163

Antibody Registry ID:

Purified: AB_2315802
TC Supernatant: AB_2315801


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

60-80 kDa depending on maturity/glycosylation


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.