Products: Primary Antibodies

Anti-AMIGO-1 Antibody (L86A/37)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-329	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-329	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Target Description:

Amphoterin-induced protein 1, amphoterin-induced gene and open reading frame, Adhesion Molecule With Ig Like Domain 1 or Amigo-1 is encoded by the gene AMIGO1 and is a member of the AMIGO family. Amigo1 acts as a neuronal adhesion protein and contains leucine-rich repeat and immunoglobin domains. It is thought to play a role in cell to cell interactions and in neuronal plasticity/regenereation. Amigo1 is expressed in the nervous system and at low levels in liver, kidney, spleen, lung and heart.

Immunogen:

Fusion protein amino acids 395-493 (cytoplasmic C-terminus) of human AMIGO-1 (also known as Amphoterin-induced protein 1, Alivin-2, Ali2, AMIGO and KIAA1163, accession number Q86WK6)
Rat: 100% identity (99/99 amino acids identical)
Mouse: 100% identity (99/99 amino acids identical)
<40% identity with AMIGO-2 and AMIGO-3

Protein Name(s):

Amphoterin-induced protein 1 (AMIGO-1) (Alivin-2)

Gene Name(s):

Amigo1 Ali2 Amigo Kiaa1163

Antibody Registry ID:

Purified: AB_2315802
TC Supernatant: AB_2315801

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

60-80 kDa depending on maturity/glycosylation

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.