Human Mouse Rat
ICC IHC WB
Amphoterin-induced protein 1, amphoterin-induced gene and open reading frame, Adhesion Molecule With Ig Like Domain 1 or Amigo-1 is encoded by the gene AMIGO1 and is a member of the AMIGO family. Amigo1 acts as a neuronal adhesion protein and contains leucine-rich repeat and immunoglobin domains. It is thought to play a role in cell to cell interactions and in neuronal plasticity/regenereation. Amigo1 is expressed in the nervous system and at low levels in liver, kidney, spleen, lung and heart.
Fusion protein amino acids 554-574 (cytoplasmic C-terminus) of human AMIGO-1 (also known as Amphoterin-induced protein 1, Alivin-2, Ali2, AMIGO and KIAA1163, accession number Q86WK6)
Rat: 100% identity (99/99 amino acids identical)
Mouse: 100% identity (99/99 amino acids identical)
<40% identity with AMIGO-2 and AMIGO-3
Amphoterin-induced protein 1 (AMIGO-1) (Alivin-2)
Amigo1 Ali2 Amigo Kiaa1163
TC Supernatant: AB_2315799
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
60-80 kDa depending on maturity/glycosylation
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.