AM1-43 Nerve Terminal Dye
Our AM1-43 Nerve Terminal Dye (equivalent to FM®1-43FX) is a fixable, activity-dependent fluorescent nerve terminal probe. It is a useful tool for synapse studies where subsequent fluorescent immunochemistry is desired.
SKU: 81-8018
Ships: 1-5 business days
Product Details
Nerve terminal dyes have highly hydrophilic, cationically charged head groups at one end with lipophilic tails at the other end. They are virtually non-fluorescent in aqueous solution, but become intensely fluorescent in membranes. Following nerve stimulation, the dye molecules are internalized in newly formed endocytic vesicles. During exocytosis, the dyes are released from the vesicles along with neurotransmitters, causing a decrease in fluorescence signal. As a result, the change in fluorescent intensity reflects the amount of endocytosis/exocytosis or synaptic activity. AM1-43 (equivalent to FM®1-43FX) is a fixable, activity-dependent fluorescent nerve terminal probe. AM1-43 functions similarly similarly to SynaptoGreen C4, but with the additional property of being fixable. Thus, AM1-43 is a useful tool for synapse studies where subsequent fluorescent immunochemistry is desired.
EM, ICC, IHC
559.5 Da
Ex: 480 nm, Em: 598 nm (in membranes)
Store at 2°C to 8°C, with desiccant; protect from light.
Solid
For use in real-time imaging of live/intact cells and cells fixed after staining in formaldehyde.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
Stable for 12 months after date of receipt.
Shipped at ambient temperature with desiccant; protected from light