Products: Primary Antibodies

Anti-Aldh1L1 (blotting) Antibody (N103/39)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-140	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $285.00
Brand	Catalog # 73-140	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $285.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC WB

Target Description:

Aldehyde dehydrogenase family 1 member L1 (Aldh1L1) is a member of the aldehyde dehydrogenase family. This protein is an enzyme that catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. It is an astrocyte marker and also is expressed in the liver. It is proposed to play a role in regulation of cellular THF levels and inhibiting cell proliferation. Alterations in Aldh1L1 have been implicated in Congenital Hydrocephalus

Immunogen:

Protein amino acids 1-902 (full-length) of rat Aldh1L1(also known as 10-formyltetrahydrofolate dehydrogenase, 10-FTHFDH, FBP-CI and Fthfd, accession number P28037); Mouse: 97% identity (875/902 amino acids identical); Human: 91% identity (823/902 amino acids identical); ~70% identity with Aldh1L2

Cross Reactivity:

No cross-reactivity against Aldh1L2

Protein Name(s):

Aldh1L1 (blotting)

Gene Name(s):

Aldh1l1 Fthfd

Antibody Registry ID:

Purified: AB_10673448
TC Supernatant: AB_10673447

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

100 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.