AEC Peroxidase Chromogen Substrate Kit

Peroxidase chromogen-substrate 3-amino-9-ethyl-carbazole (AEC) is an excellent chromogen system that is used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC).



SKU: AR-8201-01

Volume: ~400 ml reagent
Price:
Sale price$114
Peroxidase chromogen-substrate 3-amino-9-ethyl-carbazole (AEC) is an excellent chromogen system that is used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC). This chromogen system gives bright red color and is very compatible with hematoxylin nuclear counterstain. This chromogen is soluble in alcohol and organic solvents therefore cannot be mounted with organic mounting medium. We recommend our aqueous mounting medium ImmunoHistoMount (IHM), cat # AR-6503 for mounting of this chromogen.
2-8 °C
Ships overnight (domestic), International Priority Shipping
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
United States
(THIS KIT CONTAINS approximately 166X CHROMOGEN. When diluted will make approx. 425 ml))
  1. To 5 ml of distilled or deionized water in a test tube, add two drops of reagent B buffer, mix well.
  2. Add one drop of reagent C chromogen, mix well.
  3. Add one drop of reagent S substrate, mix well. This ready to use reagent is good for several hours. The unused AEC solution can be discarded according to city, county, state, province or country’s regulations.
Application: IHC, ICC
  1. After the peroxidase reaction, wash slide with buffer (this buffer should not contain any sodium azide because peroxidase is inactivated), and distilled/deionized water.
  2. Add few drops of ready to use AEC reagent, incubate at RT or 37ºC for 5-10 minutes.
  3. Wash 2-5X with buffer, followed by rinsing with distilled/deionized water.
  4. Add counterstain compatible with AEC, we use hematoxylin 30-60 seconds (cat. # AR-6521).
  5. Wash with tap water, buffer pH 7.4 or higher followed by rinsing with distilled/deionized water.
  6. Mount slide with aqueous mounting medium, we use our IHM (cat. # AR-6503) please follow the protocol for mounting medium.
  • Reagent B: Concentrated Buffer: 5 ml
  • Reagent C: Chromogen: 2.5 ml
  • Reagent: S: Substrate: 3 ml
  • Product Specific References

    PMID Publication
    33996170Park, SM, et al. 2020. Intranasal Vaccination with Outer-Membrane Protein of Orientia tsutsugamushi induces Protective Immunity Against Scrub Typhus. Immune Network, e14.
    27193041Sinha, A, et al. 2016. Circulating Gut-Homing (α4β7+) Plasmablast Responses against Shigella Surface Protein Antigens among Hospitalized Patients with Diarrhea. Clinical and Vaccine Immunology, 610-617.

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