AEC Peroxidase Chromogen Substrate Kit
Peroxidase chromogen-substrate 3-amino-9-ethyl-carbazole (AEC) is an excellent chromogen system that is used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC).
AEC Peroxidase Chromogen Substrate Kit
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Ships: 1-2 business days
(THIS KIT CONTAINS approximately 166X CHROMOGEN. When diluted will make approx. 425 ml))
- To 5 ml of distilled or deionized water in a test tube, add two drops of reagent B buffer, mix well.
- Add one drop of reagent C chromogen, mix well.
- Add one drop of reagent S substrate, mix well. This ready to use reagent is good for several hours. The unused AEC solution can be discarded according to city, county, state, province or country’s regulations.
- After the peroxidase reaction, wash slide with buffer (this buffer should not contain any sodium azide because peroxidase is inactivated), and distilled/deionized water.
- Add few drops of ready to use AEC reagent, incubate at RT or 37ºC for 5-10 minutes.
- Wash 2-5X with buffer, followed by rinsing with distilled/deionized water.
- Add counterstain compatible with AEC, we use hematoxylin 30-60 seconds (cat. # AR-6521).
- Wash with tap water, buffer pH 7.4 or higher followed by rinsing with distilled/deionized water.
- Mount slide with aqueous mounting medium, we use our IHM (cat. # AR-6503) please follow the protocol for mounting medium.
Product Specific References
| PMID | Publication |
| 33996170 | Park, SM, et al. 2020. Intranasal Vaccination with Outer-Membrane Protein of Orientia tsutsugamushi induces Protective Immunity Against Scrub Typhus. Immune Network, e14. |
| 27193041 | Sinha, A, et al. 2016. Circulating Gut-Homing (α4β7+) Plasmablast Responses against Shigella Surface Protein Antigens among Hospitalized Patients with Diarrhea. Clinical and Vaccine Immunology, 610-617. |