Products: Primary Antibodies

Anti-6xHis Antibody (N144/14)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-169
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$275.00
Brand
NeuroMab Logo
Catalog #
73-169
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$275.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ELISA ICC WB

Target Description:

6xHIS or His-tag is a commonly used recombinant tag that consists of 6 sequential histidine residues placed at the amino or carboxy terminus of a targeted sequence. 6xHis is useful for purification of proteins or visualization by western blot or immunocytochemistry.

Immunogen:

Identified as off-target mAb in screen for anti-ProtocadherinGammaA3 Neuromabs (immunogen was 6xHis fusion protein amino acids 720-804, variable portion of cytoplasmic C-terminus, of mouse ProtocadherinGammaA3). Target identified as 6xHis by ELISA, immunofluorescence and immunoblot against other 6xHis-tagged fusion proteins.

Cross Reactivity:

No cross-reactivity reported

Protein Name(s):

6xHis

Gene Name(s):

N/A

Antibody Registry ID:

Purified: AB_10673446
TC Supernatant: AB_10671171


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

None Reported


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.