ELISA ICC WB
6xHIS or His-tag is a commonly used recombinant tag that consists of 6 sequential histidine residues placed at the amino or carboxy terminus of a targeted sequence. 6xHis is useful for purification of proteins or visualization by western blot or immunocytochemistry.
Identified as off-target mAb in screen for anti-ProtocadherinGammaA3 Neuromabs (immunogen was 6xHis fusion protein amino acids 720-804, variable portion of cytoplasmic C-terminus, of mouse ProtocadherinGammaA3). Target identified as 6xHis by ELISA, immunofluorescence and immunoblot against other 6xHis-tagged fusion proteins.
No cross-reactivity reported
TC Supernatant: AB_10671171
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.