ELISA ICC WB
Identified as off-target mAb in screen for anti-ProtocadherinGammaA3 Neuromabs (immunogen was 6xHis fusion protein amino acids 720-804, variable portion of cytoplasmic C-terminus, of mouse ProtocadherinGammaA3). Target identified as 6xHis by ELISA, immunofluorescence and immunoblot against other 6xHis-tagged fusion proteins.
TC Supernatant: AB_10671171
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.
Citations and References
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- Sun Z, Runne C, Tang X, Lin F, Chen S.. (2012), 'The Gβ3 splice variant associated with the C825T gene polymorphism is an unstable and functionally inactive protein. .' Cellular Signalling. 10.1016/j.cellsig.2012.08.011. " "
- Li Y, Xiao H, Chiou TT, Jin H, Bonhomme B, Miralles CP, Pinal N, Ali R, Chen WV, Maniatis T, De Blas AL.. (2012), 'Molecular and functional interaction between protocadherin-γC5 and GABAA receptors. .' Journal of Neuroscience. 10.1523/JNEUROSCI.0969-12.2012. " "
- Emond MR, Biswas S, Blevins CJ, Jontes JD.. (2011), 'A complex of Protocadherin-19 and N-cadherin mediates a novel mechanism of cell adhesion. .' The Journal of Biology. 10.1083/jcb.201108115. " "
- Hsu S, Kim Y, Li S, Durrant ES, Pace RM, Woods VL Jr, Gentry MS.. (2009), 'Structural insights into glucan phosphatase dynamics using amide hydrogen-deuterium exchange mass spectrometry. .' Biochemistry . 10.1021/bi9008853. "