Products: Primary Antibodies

Anti-6xHis (N144/14)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-169	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-169	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Applications:

ELISA ICC WB

Immunogen:

Identified as off-target mAb in screen for anti-ProtocadherinGammaA3 Neuromabs (immunogen was 6xHis fusion protein amino acids 720-804, variable portion of cytoplasmic C-terminus, of mouse ProtocadherinGammaA3). Target identified as 6xHis by ELISA, immunofluorescence and immunoblot against other 6xHis-tagged fusion proteins.

Cross Reactivity:

None Reported

Protein Name(s):

N/A

Gene Name(s):

N/A

Antibody Registry ID:

Purified: AB_10673446
TC Supernatant: AB_10671171

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Expected Banding Pattern:

None Reported

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.

Citations and References

Donati RJ,, Schappi J, Czysz AH, Jackson A, Rasenick MM.. (2016), 'Differential effects of antidepressants escitalopram versus lithium on Gs alpha membrane relocalization. .' BMC Neuroscience. 10.1186/s12868-015-0178-y.
Yokoi N, Fukata Y, Kase D, Miyazaki T, Jaegle M, Ohkawa T, Takahashi N, Iwanari H, Mochizuki Y, Hamakubo T, Imoto K, Meijer D, Watanabe M, Fukata M.. (2015), 'Chemical corrector treatment ameliorates increased seizure susceptibility in a mouse model of familial epilepsy. .' Nature Medicine. 10.1038/nm.3759.
Erickson AI, Sarsam RD, Fisher AJ.. (2014), 'Expression, purification and preliminary crystallographic analysis of Mycobacterium tuberculosis CysQ, a phosphatase involved in sulfur metabolism. .' (IUCr) Acta Crystallographica Section F. 10.1107/S2053230X14008619.
Benoit RM, Frey D, Hilbert M, Kevenaar JT, Wieser MM, Stirnimann CU, McMillan D, Ceska T, Lebon F, Jaussi R, Steinmetz MO, Schertler GF, Hoogenraad CC, Capitani G, Kammerer RA.. (2014), 'Structural basis for recognition of synaptic vesicle protein 2C by botulinum neurotoxin A. .' Nature. 10.1038/nature12732.
Barry J, Xu M, Gu Y, Dangel AW, Jukkola P, Shrestha C, Gu C.. (2013), 'Activation of conventional kinesin motors in clusters by Shaw voltage-gated K+ channels. .' Journal of Cell Science. 10.1242/jcs.122234.
Sun Z, Runne C, Tang X, Lin F, Chen S.. (2012), 'The Gβ3 splice variant associated with the C825T gene polymorphism is an unstable and functionally inactive protein. .' Cellular Signalling. 10.1016/j.cellsig.2012.08.011.
Li Y, Xiao H, Chiou TT, Jin H, Bonhomme B, Miralles CP, Pinal N, Ali R, Chen WV, Maniatis T, De Blas AL.. (2012), 'Molecular and functional interaction between protocadherin-γC5 and GABAA receptors. .' Journal of Neuroscience. 10.1523/JNEUROSCI.0969-12.2012.
Emond MR, Biswas S, Blevins CJ, Jontes JD.. (2011), 'A complex of Protocadherin-19 and N-cadherin mediates a novel mechanism of cell adhesion. .' The Journal of Biology. 10.1083/jcb.201108115.
Hsu S, Kim Y, Li S, Durrant ES, Pace RM, Woods VL Jr, Gentry MS.. (2009), 'Structural insights into glucan phosphatase dynamics using amide hydrogen-deuterium exchange mass spectrometry. .' Biochemistry . 10.1021/bi9008853.