SfN 2024 Free Antibody Promotion

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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit phosphorylated at Tyr1252 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).Immunostaining of 14 DIV rat cortical neurons showing NR2B phosphorylated at Tyr1252 (red, 1:400) and PSD95 (green). Photo courtesy of Gang Liu.
PhosphoSolutions Anti-NMDA NR2B Subunit (Tyr1252) Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit phosphorylated at Tyr1336 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min). Immunostaining of mouse dentate gyrus 48 hours post TMT treatment showing NR2B when phosphorylated at Tyr1336 (green, 1:400). The blue is staining nuclei using DAPI. Photo Courtesy of Rob Wine.
PhosphoSolutions Anti-NMDA NR2B Subunit (Tyr1336) Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Tyr1472 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).
PhosphoSolutions Anti-NMDA NR2B Subunit (Tyr1472) Antibody
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Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.
PhosphoSolutions Anti-NMDA NR2B Subunit Antibody
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Immunofluorescence of healthy iPSC-derived neurons labeled with Anti-NMDA Receptor, NR2B subunit (Cat. 1498-NR2B, green, 1:100) and Anti-TuJ1 (red). The blue is Hoechst staining of nuclear DNA. Image kindly provided by Dr. Allison Ebert, Medical College of Wisconsin.Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.
PhosphoSolutions Anti-NMDA NR2B Subunit Antibody
$385
Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.
PhosphoSolutions Anti-NMDA NR2B Subunit Antibody
$445
Immunostaining of 14 DIV rat cortical neurons showing NR2B (green, 1:400) and PSD95(red). Photo courtesy of Gang Liu. Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.
PhosphoSolutions Anti-NMDA NR2B Subunit, N-terminus Antibody
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Western blot of mouse brain lysate showing specific immunolabeling of the ~140 kDa NR2C subunit of the NMDA receptor phosphorylated at Ser1096 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-NMDA NR2C Subunit (Ser1096) Antibody
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Western blot of rat cerebellar lysate showing specific immunolabeling of the ~140 kDa NR2C subunit of the NMDA receptor phosphorylated at Ser1244 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-NMDA NR2C Subunit (Ser1244) Antibody
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Western blot of 10 µg of rat cerebellar lysate showing specific immunolabeling of the ~140 kDa NR2C subunit of the NMDA receptor.
PhosphoSolutions Anti-NMDA NR2C Subunit Antibody
$445
Western blot of rat striatal lysate showing specific labeling of the ~75 kDa NSF protein.
PhosphoSolutions Anti-NSF (N-ethylmaleimide Sensitive Fusion Protein) Antibody
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Western blot of adult mouse brain. The blots were probed with anti-nSMase2 (C-terminal region) rabbit polyclonal antibody at 1:2000 (lane 1), 1:1000 (lane 2), or 1:500 in the absence (lane 3) or presence of nSMase2 blocking peptide (SX4065) (lane 4).Immunocytochemical labeling of nSMase2 in aldehyde-fixed and NP-40-permeabilized differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-nSMase2 (SP4061) antibody in the absence (Left) or presence (Right) of blocking peptide (SX4065). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. The cells were counterstained with Sytox green to label nuclei.
PhosphoSolutions Anti-nSMase2 (C-terminal region) Antibody
$340
Western blot of nSMase3a and nSMase3b GFP transfectants (A) and nSMase3 siRNA knockdown in C2C12 myotubes (B). Both blots were probed with anti-nSMase3 (C-terminal region) rabbit polyclonal antibody (SP0281). (Image provided by Dr. Jennifer Moylan, Dept. Physiology, University of Kentucky).Immunocytochemical labeling of nSMase3 in aldehyde-fixed and NP-40-permeabilized mouse C2C12 cells. The cells were labeled with rabbit polyclonal anti-nSMase3 (SP0281) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-nSMase3 (C-terminal region) Antibody
$340
Immunostaining of HeLa cells showing specific labeling of nuclei using the anti-nuclei antibody (Cat. 1590-NUC, 1:100, green).
PhosphoSolutions Anti-Nuclei Antibody
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Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~ 27 kDa Olig1 protein.Immunostaining of primary rat oligodendrocytes using anti-Olig1 (Cat. 1537-OLIG1, red, 1:100).
PhosphoSolutions Anti-Olig1 Antibody
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Western blot of rat neonatal brain lysate showing specific immunolabeling of the ~32 kDa form of the Olig2 protein phosphorylated at Ser10,13,14 in the first lane (-). Phosphospecificity is shown in the second lane (+) where Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-Olig2 (Ser10/13/14) Antibody
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Western blot analysis of human recombinant osteopontin protein (lane 1 & 2) or human MDA-MB-231 cells (lane 3 & 4). The blots were probed with mouse monoclonal anti-Osteopontin (OM5741) at a dilution of 1:1000 (lane 1), 1:4000 (lane 2), 1:250 (lane 3), and 1:1000 (lane 4).Immunocytochemical labeling of Osteopontin in paraformaldehyde fixed and NP-40 permeabilized MCF-7 cells. The cells were labeled with mouse monoclonal anti-Osteopontin (M574). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-Osteopontin (N-terminal region) Antibody
$340
Western blot analysis of human endothelial cells serum starved overnight (lanes 1, 3, & 5) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4, & 6). The blot was probed with anti-p130 Cas (PM1441; lanes 1 & 2), anti-p130 Cas (Tyr-751) (PP1581; lanes 3 & 4) or anti-p130 Cas (Tyr-762) (PP1451; lanes 5 & 6).
PhosphoSolutions Anti-p130 Cas (Tyr-751), Phosphospecific Antibody
$340
Western blot analysis of human endothelial cells serum starved overnight (lanes 1, 3, & 5) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4, & 6). The blot was probed with anti-p130 Cas (PM1441; lanes 1 & 2), anti-p130 Cas (Tyr-751) (PP1581; lanes 3 & 4) or anti-p130 Cas (Tyr-762) (PP1451; lanes 5 & 6).
PhosphoSolutions Anti-p130 Cas (Tyr-762), Phosphospecific Antibody
$340
Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-p38α (lanes 1 & 2) or anti-p38 (T180/Y182) (lanes 3-4). Lanes 5-7 shows a blot of A431 cells treated with pervanadate and probed with anti-p38 (T180/Y182) in the presence of no peptide (lane 5), phospho-ERK1 (T202/Y204) peptide (lane 6) or phospho-p38 (T180/Y182) peptide (lane 7).Immunocytochemical labeling of activated p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38α MAPK and p38 MAPK (T180/Y182) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-p38 MAP Kinase (Thr-180/Tyr-182), Phosphospecific Antibody
$370
Western blot of HeLa cell lysates that had been treated with UV (~254 nm) for 0’ or 30’ showing the specific immunolabeling of the ~39 kDa p38 MAPK protein phosphorylated at Thr180/Tyr182.Immunostaining of human breast cancer tissue showing p38 when phosphorylated at Thr180/Tyr182 (red, 1:250). Photo courtesy of Patsy Ruegg.
PhosphoSolutions Anti-p38 MAPK (Thr180/Tyr182) Antibody
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Western blot analysis of p38 MAP kinase in mouse macrophage (J774A.1) cell lysate (lanes 1-4). The blots were probed with mouse monoclonal anti-p38α (C-terminal) at 1:500 (lane 1) and 1:2000 (lane 2) or rabbit polyclonal anti-p38α (a.a. 319-328) at 1:250 (lane 3) and 1:1000 (lane 4).
PhosphoSolutions Anti-p38α MAP Kinase (C-terminal) Antibody
$285
Western blot analysis of human A431 cells (lanes 1 & 2) and rat A7r5 cells (lanes 3 & 4). The blot was probed with mouse monoclonal anti-p38α (C-terminal) clone M548 at a dilution of 1:250 (lanes 1 & 3) or 1:1000 (lanes 2 & 4).Immunocytochemical labeling of p38α in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-p38α (PM5481). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-p38α MAP Kinase (C-terminal) Antibody
$285
Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-p38α (lanes 1 & 2) or anti-p38 (T180/Y182) (lanes 3-4). Lanes 5-7 shows a blot of A431 cells treated with pervanadate and probed with anti-p38 (T180/Y182) in the presence of no peptide (lane 5), phospho-ERK1 (T202/Y204) peptide (lane 6) or phospho-p38 (T180/Y182) peptide (lane 7).Immunocytochemical labeling of activated p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38α MAPK and p38 MAPK (T180/Y182) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-p38α MAP Kinase (C-terminal) M138 Antibody
$370

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