Our WWP1 (N-terminus) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat WWP1 (N-terminus) and is antigen affinity purified. It is great for use in WB.
Western blot analysis of WWP1 expression in adult mouse liver (lane 1) and human prostate adenocarcinoma (PC3) cells (lane 2). The blot was probed with anti-WWP1 (WP3931) at 1:1000.
The Nedd4-like family of E3 ubiquitin ligases have been implicated in several types of human cancer. There are nine members of the Nedd4-like E3 family, all of which have an N-terminal C2 domain, two to four WW domains in the central region, and a C-terminal domain that is homologous to the C-terminus of E6-AP. This ubiquitin ligase family includes NEDD4-1, NEDD4-2, AIP4/Itch, AIP5/WWP1, SMURF1 and SMURF2. Several ubiquitin ligases in this family are overexpressed in human cancers. WWP1 has been implicated as an oncogene as it is overexpressed in prostate and breast cancers, and knockdown of this ligase reduces cell proliferation and causes apoptosis. In addition, WWP1 has been shown to negatively regulate transforming growth factor-β (TGFβ) signaling by targeting TGFβ receptor 1, Smad2, and Smad4 for ubiquitin-mediated degradation. WWP1 has also been shown to regulate the protein levels of the epithelial sodium channel (ENaC), Notch, EGFR/ErbB2, Runx2, KLF2, KLF5, p53, and p63. Thus, WWP1 may regulate cell development and proliferation through the degradation of proteins involved in a variety of cell signaling pathways.
Antigen Affinity Purified
Polyclonal
IgG
ELISA, WB
Rabbit
WWP1
110
WWP1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the N-terminus of human WWP1. This sequence is highly conserved in rat and mouse WWP1, and has low homology to other NEDD4-like family members.
Human
Chicken, Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
Unconjugated
The antibody detects a 110 kDa* protein corresponding to the apparent molecular mass of WWP1 on SDS-PAGE immunoblots of adult mouse liver and human PC3 cells. The antibody also detects lower molecular weight isoforms of WWP1 in mouse liver, and human PC3, HepG2, K562, HeLa, and HUVEC.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Q9H0M0
UniProt Summary: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1; the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF. Activates the Hippo signaling pathway in response to cell contact inhibition and recruitment to the Crumbs complex at the cell membrane. Monoubiquitinates AMOTL2 which facilitates its interaction with and activation of LATS2. LATS2 then phosphorylates YAP1, excluding it from the nucleus and therefore ultimately represses YAP1-driven transcription of target genes.
11059
Blue Ice
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